Wang Yulan, Tang Huiru, Holmes Elaine, Lindon John C, Turini Marco E, Sprenger Norbert, Bergonzelli Gabriela, Fay Laurent B, Kochhar Sunil, Nicholson Jeremy K
Biological Chemistry, Biomedical Sciences Division, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London, SW7, 2AZ, United Kingdom.
J Proteome Res. 2005 Jul-Aug;4(4):1324-9. doi: 10.1021/pr050032r.
We report details of metabolic profiles for small intestinal samples obtained using high-resolution magic-angle-spinning (HRMAS) (1)H NMR spectroscopy. Intact samples of jejunum and ileum from male Long Evans rats were analyzed on a 600 MHz spectrometer using standard one and two-dimensional (1)H NMR spectroscopic pulse sequences. The metabolic profiles of ileum and jejunum predominantly comprised a number of amino acids, lipids, glycerophosphocholine (GPC), choline, creatine, and ethanol, a number of carboxylic acids including acetate and lactate, and nucleoside bases including cytosine, isocytosine, and uracil. Principal component analysis (PCA) was applied to these NMR data to characterize the biochemical differences between jejunum and ileum tissues. Compared with ileum, jejunum contained higher levels of lipids, GPC, choline, lactate and creatinine, but lower levels of amino acids and acetate. In addition, the age dependence of the biochemical composition of intestinal tissues from young rats (15, 36 days and 3-4 months old) was studied. In general, levels of lipids, lactate, taurine and creatinine were positively correlated with age while amino acids and GPC decreased in the older age group. This study will provide a metabolic reference for further studies assessing the metabolic consequences of nutrition, stress and gut microbiota on intestinal composition.
我们报告了使用高分辨率魔角旋转（HRMAS）氢核磁共振（¹H NMR）光谱法获得的小肠样本代谢谱的详细信息。对雄性Long Evans大鼠的空肠和回肠完整样本，在600 MHz光谱仪上使用标准的一维和二维¹H NMR光谱脉冲序列进行分析。回肠和空肠的代谢谱主要包括多种氨基酸、脂质、甘油磷酸胆碱（GPC）、胆碱、肌酸和乙醇，多种羧酸（包括乙酸盐和乳酸盐），以及核苷碱基（包括胞嘧啶、异胞嘧啶和尿嘧啶）。对这些NMR数据应用主成分分析（PCA）来表征空肠和回肠组织之间的生化差异。与回肠相比，空肠中脂质、GPC、胆碱、乳酸和肌酐的含量较高，但氨基酸和乙酸盐的含量较低。此外，还研究了幼鼠（15天、36天和3 - 4个月大）肠道组织生化组成的年龄依赖性。总体而言，脂质、乳酸、牛磺酸和肌酐的水平与年龄呈正相关，而老年组中氨基酸和GPC含量下降。本研究将为进一步评估营养、应激和肠道微生物群对肠道组成的代谢影响的研究提供代谢参考。
