Maiorano Victor A, Marcussi Silvana, Daher Maristela A F, Oliveira Clayton Z, Couto Lucélio B, Gomes Odair A, França Suzelei C, Soares Andreimar M, Pereira Paulo S
Unidade de Biotecnologia, Universidade de Ribeirão Preto (UNAERP), SP, Brazil.
J Ethnopharmacol. 2005 Dec 1;102(3):364-70. doi: 10.1016/j.jep.2005.06.039. Epub 2005 Aug 3.
Aqueous extracts, prepared from dried or fresh roots, stems or leaves of Mikania glomerata, a plant found in Mata Atlântica in Southeastern Brazil, were able to efficiently neutralize different toxic, pharmacological, and enzymatic effects induced by venoms from Bothrops and Crotalus snakes. Phospholipase A(2) activity and the edema induced by Crotalus durissus terrificus venom were inhibited around 100 and approximately 40%, respectively, although this inhibition was only partial for Bothrops venoms. The hemorrhagic activity of Bothrops venoms (Bothrops altenatus, Bothrops moojeni, Bothrops neuwiedi, and Bothrops jararacussu) was significantly inhibited by this vegetal species, while the clotting activity of Crotalus durissus terrificus, Bothrops jararacussu, and Bothrops neuwiedi venoms was totally inhibited. Although, the mechanism of action of Mikania glomerata extract is still unknown, the finding that no visible change was detected in the electrophoretic pattern of snake venom after incubation with the extract excludes proteolytic degradation as a potential mechanism. Since the extract of Mikania glomerata significantly inhibited the studied snake venoms, it may be used as an alternative treatment to serumtherapy and, in addition, as a rich source of potential inhibitors of PLA(2)s, metalloproteases and serineproteases, enzymes involved in several physiopathological human and animal diseases.
从巴西东南部大西洋森林中发现的植物薇甘菊的干燥或新鲜根、茎或叶制备的水提取物，能够有效中和矛头蝮属和响尾蛇属蛇毒诱导的不同毒性、药理和酶促作用。虽然对矛头蝮属蛇毒的抑制只是部分抑制，但对杜氏响尾蛇毒诱导的磷脂酶A(2)活性和水肿分别抑制了约100%和40%。薇甘菊这种植物能显著抑制矛头蝮属蛇毒（交替矛头蝮、穆氏矛头蝮、纽氏矛头蝮和巴西矛头蝮）的出血活性，同时完全抑制杜氏响尾蛇、巴西矛头蝮和纽氏矛头蝮蛇毒的凝血活性。虽然薇甘菊提取物的作用机制尚不清楚，但提取物与蛇毒孵育后蛇毒电泳图谱未检测到可见变化这一发现排除了蛋白水解降解作为潜在机制。由于薇甘菊提取物显著抑制所研究的蛇毒，它可作为抗蛇毒血清疗法的替代治疗方法，此外，还可作为磷脂酶A2、金属蛋白酶和丝氨酸蛋白酶潜在抑制剂的丰富来源，这些酶参与多种人类和动物生理病理疾病。
