紫羊蹄甲水提取物对蛇毒的抗凝和抗纤维蛋白溶解特性。

Anticoagulant and antifibrinogenolytic properties of the aqueous extract from Bauhinia forficata against snake venoms.

作者信息

Oliveira Clayton Z, Maiorano Victor A, Marcussi Silvana, Sant'ana Carolina D, Januário Ana H, Lourenço Miriam V, Sampaio Suely V, França Suzelei C, Pereira Paulo S, Soares Andreimar M

机构信息

Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP, 14096-900 Ribeirão Preto, SP, Brazil.

出版信息

J Ethnopharmacol. 2005 Apr 8;98(1-2):213-6. doi: 10.1016/j.jep.2004.12.028.

DOI:10.1016/j.jep.2004.12.028

PMID:15763387

Abstract

The aqueous extract from aerial parts of Bauhinia forficata was able to neutralize the clotting activity induced by Bothrops and Crotalus crude venoms. The clotting time, upon human plasma, induced by B. moojeni venom was significantly prolonged. Clotting and fibrinogenolytic activities induced by isolated thrombin-like enzyme from Bothrops jararacussu were totally inhibited after incubation at different ratios. The extract was not able to neutralize the hemorrhagic activity induced by an Bothrops venoms, but it efficiently inhibited the edema induced by Crotalus durissus terrificus venom and isolated PLA2s. In addition, it did not inhibited the phospholipase A2 activity of Bothrops snake venoms. Interaction studies between Bauhinia forficata extract and snake venoms, when analyzed by SDS-PAGE, did not reveal any apparent degradation of the venom proteins. This extract is a promising source of natural inhibitors of serine-proteases involved in blood clotting disturbances induced by snake venoms.

摘要

羊蹄甲地上部分的水提取物能够中和矛头蝮属和响尾蛇属粗毒诱导的凝血活性。由穆氏矛头蝮毒液诱导的人血浆凝血时间显著延长。不同比例孵育后，巴西矛头蝮分离出的类凝血酶诱导的凝血和纤维蛋白溶解活性被完全抑制。该提取物不能中和矛头蝮属毒液诱导的出血活性，但能有效抑制杜氏响尾蛇毒液和分离的磷脂酶A2诱导的水肿。此外，它不抑制矛头蝮属蛇毒的磷脂酶A2活性。通过SDS-PAGE分析羊蹄甲提取物与蛇毒之间的相互作用，未发现毒液蛋白有明显降解。该提取物是参与蛇毒诱导的凝血紊乱的丝氨酸蛋白酶天然抑制剂的一个有前景的来源。