Luongo Marina, Porta Amalia, Maresca Bruno
Laboratory of Molecular Fungal Pathogenesis, Institute of Genetics and Biophysics Buzzati Traverso, Naples, Italy.
FEMS Immunol Med Microbiol. 2005 Sep 1;45(3):471-8. doi: 10.1016/j.femsim.2005.06.007.
During macrophage infection Candida albicans expresses differentially several genes whose functions are associated with its survival strategy. Among others, we have isolated CaGS gene, which is homologous to SNF3, a glucose sensor of Saccharomyces cerevisiae. To elucidate its potential role during infection, CaGS has been disrupted and the resulting phenotype analyzed on different solid media. The null mutant lost the ability to form hyphae on a medium with low glucose concentration and serum. Furthermore, this mutant does not disrupt macrophage in in vitro infections. We believe that this putative glucose sensor is involved in hyphal development during macrophage infection.
在巨噬细胞感染期间，白色念珠菌会差异表达几个与其生存策略相关的基因。其中，我们分离出了CaGS基因，它与酿酒酵母的葡萄糖传感器SNF3同源。为了阐明其在感染过程中的潜在作用，CaGS基因已被破坏，并在不同的固体培养基上分析了产生的表型。缺失突变体在低葡萄糖浓度和血清的培养基上失去了形成菌丝的能力。此外，该突变体在体外感染中不会破坏巨噬细胞。我们认为这个假定的葡萄糖传感器参与了巨噬细胞感染期间的菌丝发育。
