Yáñez Jaime A, Miranda Nicole D, Remsberg Connie M, Ohgami Yusuke, Davies Neal M
College of Pharmacy, Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program, Washington State University, Pullman, WA 99164-6534, USA.
J Pharm Biomed Anal. 2007 Jan 4;43(1):255-62. doi: 10.1016/j.jpba.2006.06.027. Epub 2006 Jul 31.
A stereospecific method of analysis of eriodictyol [5,7,3',4'-tetrahydroxyflavanone] in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism, and tissue distribution in fruits and humans. A simple high-performance liquid chromatographic method was developed for the stereospecific determination of eriodictyol in rat and human urine. Separation was achieved on a Chiralpak OJ-RH column with UV detection at 288 nm. The stereospecific calibration curves were linear ranging from 0.5 to 100 microg/ml. The mean extraction efficiency was >98.8%. Precision of the assay was <15% (CV), and was within 12% at the limit of quantitation (0.5 microg/ml). Bias of the assay was lower than 8%, and was within 6% at the limit of quantitation. The assay was applied successfully to the urinary excretion of eriodictyol in rats and humans, and to the stereospecific quantification of eriodictyol in raw lemon juice, conventional and organic lemonade.
为了研究体外和体内代谢动力学以及水果和人体中的组织分布，需要一种用于分析生物流体中圣草酚[5,7,3',4'-四羟基黄烷酮]的立体特异性方法。开发了一种简单的高效液相色谱法，用于立体特异性测定大鼠和人尿液中的圣草酚。在Chiralpak OJ-RH柱上进行分离，于288 nm处进行紫外检测。立体特异性校准曲线在0.5至100微克/毫升范围内呈线性。平均提取效率>98.8%。该测定法的精密度<15%（变异系数），在定量限（0.5微克/毫升）时精密度在12%以内。该测定法的偏差低于8%，在定量限时偏差在6%以内。该测定法已成功应用于大鼠和人体中圣草酚的尿排泄，以及生柠檬汁、传统柠檬水和有机柠檬水中圣草酚的立体特异性定量。
