Nakayama Seiko, Mukae Hiroshi, Ishii Hiroshi, Kakugawa Tomoyuki, Sugiyama Kanako, Sakamoto Noriho, Fujii Takeshi, Kadota Jun-Ichi, Kohno Shigeru
Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan.
Respir Med. 2005 Sep;99(9):1145-51. doi: 10.1016/j.rmed.2005.02.021. Epub 2005 Mar 25.
Epithelial neutrophil-activating peptide 78 (ENA-78) and interferon gamma-inducible protein 10 (IP10) belong to the CXC chemokine family and are considered to be important factors in idiopathic pulmonary fibrosis (IPF). Idiopathic nonspecific interstitial pneumonia (NSIP) and IPF are the two largest subsets of idiopathic interstitial pneumonias (IIP). In patients with NSIP, the prognosis is generally good compared with IPF. Therefore, the pathogenesis of NSIP seems to be different from that of IPF, but this remains unclear. The aim of the present study was to evaluate the contribution of ENA-78 and IP10 in the two diseases.
We measured the levels of ENA-78 and IP10 in serum and bronchoalveolar lavage fluid (BALF) of patients with IPF (n=17), idiopathic NSIP (n=10) and healthy subjects (n=12) by enzyme-linked immunosorbent assays.
The level of ENA-78 in BALF was significantly higher in IPF patients than in NSIP patients and controls. Serum levels of ENA-78 and BALF levels of IP10 in NSIP patients were significantly higher than in patients with IPF and controls. In BALF of patients with NSIP, IP10 level significantly correlated with the absolute number of lymphocytes. In IPF patients, BALF IP10 levels also correlated with the proportion of lymphocytes in BALF.
Our results show distinct profiles of CXC chemokines in IPF and NSIP, and suggest that these chemokines play an important role in inflammatory cell recruitment into the lung in patients with IIP.
上皮中性粒细胞激活肽78（ENA - 78）和干扰素γ诱导蛋白10（IP10）属于CXC趋化因子家族，被认为是特发性肺纤维化（IPF）的重要因素。特发性非特异性间质性肺炎（NSIP）和IPF是特发性间质性肺炎（IIP）的两个最大亚组。与IPF患者相比，NSIP患者的预后通常较好。因此，NSIP的发病机制似乎与IPF不同，但尚不清楚。本研究的目的是评估ENA - 78和IP10在这两种疾病中的作用。
我们通过酶联免疫吸附测定法测量了IPF患者（n = 17）、特发性NSIP患者（n = 10）和健康受试者（n = 12）血清和支气管肺泡灌洗液（BALF）中ENA - 78和IP10的水平。
IPF患者BALF中ENA - 78水平显著高于NSIP患者和对照组。NSIP患者血清ENA - 78水平和BALF中IP10水平显著高于IPF患者和对照组。在NSIP患者的BALF中，IP10水平与淋巴细胞绝对数显著相关。在IPF患者中，BALF中IP10水平也与BALF中淋巴细胞比例相关。
我们的结果显示了IPF和NSIP中CXC趋化因子的不同特征，并表明这些趋化因子在IIP患者肺部炎症细胞募集中起重要作用。
