Department of Pneumology and Allergology, Ruhrlandklinik, University Hospital, University Duisburg-Essen, Tueschener Weg 40, D-45239 Essen, Germany.
Respir Med. 2013 Sep;107(9):1444-52. doi: 10.1016/j.rmed.2013.06.004. Epub 2013 Jul 5.
CCL18 is a CC chemokine produced mainly by antigen-presenting cells, and is chemotactic predominantly for T-lymphocytes. CCL18 can stimulate pulmonary fibroblasts and increase the collagen production in vitro.
This study aimed to compare the CCL18 levels in a variety of human biological fluids between various interstitial lung diseases (ILDs), and to reveal potential correlations with BAL cell differentials.
Serum and bronchoalveolar lavage fluid (BALF) samples were collected from 199 patients with idiopathic pulmonary fibrosis (IPF), idiopathic non-specific interstitial pneumonia (iNSIP), respiratory bronchiolitis interstitial lung disease/desquamative interstitial pneumonia (RB-ILD/DIP), cryptogenic organizing pneumonia (COP), hypersensitivity pneumonitis (HP) or sarcoidosis. Alveolar macrophage (AM) culture was performed in 44 patients with IPF, iNSIP, COP, HP, sarcoidosis or non-ILDs. The CCL18 levels in serum, BALF and AM culture supernatant were measured with ELISA.
Both serum and BALF CCL18 levels in all ILDs were higher than in controls (all p < 0.005). In HP, CCL18 serum levels were the highest of all ILDs, and its BALF levels were significantly higher than in other ILDs except iNSIP. The BALF CCL18 levels markedly correlated with BAL cell differentials, especially with the percentage of BAL lymphocytes. In AM culture supernatant, the spontaneous CCL18 production was higher in HP and COP than in IPF and controls.
CCL18 levels in serum, BALF and AM culture supernatant are markedly increased in various inflammatory and fibrotic ILDs. However, the CCL18 level being highest in HP among the investigated ILDs suggests that CCL18 may be more profoundly involved in inflammatory immune responses.
CCL18 是一种主要由抗原呈递细胞产生的 CC 趋化因子，主要趋化 T 淋巴细胞。CCL18 可刺激肺成纤维细胞并增加体外胶原的产生。
本研究旨在比较各种间质性肺疾病（ILD）患者血清和支气管肺泡灌洗液（BALF）中 CCL18 水平，并揭示与 BAL 细胞分类的潜在相关性。
收集了 199 例特发性肺纤维化（IPF）、特发性非特异性间质性肺炎（iNSIP）、呼吸性细支气管炎间质性肺病/脱屑性间质性肺炎（RB-ILD/DIP）、隐源性机化性肺炎（COP）、过敏性肺炎（HP）或结节病患者的血清和 BALF 样本。对 44 例 IPF、iNSIP、COP、HP、结节病或非 ILD 患者进行肺泡巨噬细胞（AM）培养。用 ELISA 法检测血清、BALF 和 AM 培养上清液中 CCL18 水平。
所有 ILD 患者的血清和 BALF CCL18 水平均高于对照组（均 p<0.005）。在 HP 中，CCL18 血清水平在所有 ILD 中最高，其 BALF 水平除 iNSIP 外，均显著高于其他 ILD。BALF CCL18 水平与 BAL 细胞分类明显相关，尤其是与 BAL 淋巴细胞百分比相关。在 AM 培养上清中，HP 和 COP 中 AM 自发产生的 CCL18 高于 IPF 和对照组。
各种炎症性和纤维化性 ILD 患者的血清、BALF 和 AM 培养上清液中的 CCL18 水平明显升高。然而，在所研究的 ILD 中，HP 中 CCL18 水平最高，提示 CCL18 可能更深入地参与炎症免疫反应。
