葡萄膜黑色素瘤中的浸润淋巴细胞和抗原表达

Infiltrating lymphocytes and antigen expression in uveal melanoma.

作者信息

Meecham W J, Char D H, Kaleta-Michaels S

机构信息

Department of Ophthalmology, University of California, San Francisco 49143-0730.

出版信息

Ophthalmic Res. 1992;24(1):20-6. doi: 10.1159/000267140.

DOI:10.1159/000267140

PMID:1608588

Abstract

Monoclonal antibodies and flow cytometry were used to study lymphocyte, monocyte and tumor cell antigen expression in uveal melanoma. Forty-one melanomas were studied after various forms of treatment. An antimelanoma antibody, 13A3E, stained 81.7% of the cells. Tumors treated with helium ions or 125I plaques had 13A3E staining reduced to 62.5%, p = 0.011. HLA-A,B,C stained 85.4%, and HLA-DR stained 7.0% of the cells. T cells (CD3 positive) comprised 4.5% (range 0.1-29.2%) of total cell population. Natural killer (NK) cells, B cells and macrophages were present in small numbers (mean less than 2.5% in each case). Tumors with numerous T cells (greater than 5%), were used for T cell subtype studies. The mean helper/inducer (CD4) to cytotoxic/suppressor (CD8) ratio was 1.00 (range 0.11-3.15). CD8 decreased, and CD4 increased with age in unirradiated tumors (p less than 0.02).

摘要

采用单克隆抗体和流式细胞术研究葡萄膜黑色素瘤中淋巴细胞、单核细胞和肿瘤细胞的抗原表达。对41例黑色素瘤进行了各种形式治疗后进行研究。一种抗黑色素瘤抗体13A3E可使81.7%的细胞染色。经氦离子或碘-125斑块治疗的肿瘤，13A3E染色降至62.5%，p = 0.011。HLA-A、B、C染色阳性率为85.4%，HLA-DR染色阳性率为7.0%。T细胞（CD3阳性）占总细胞群的4.5%（范围0.1 - 29.2%）。自然杀伤（NK）细胞、B细胞和巨噬细胞数量较少（每种情况平均少于2.5%）。对T细胞数量较多（大于5%）的肿瘤进行T细胞亚群研究。辅助/诱导（CD4）与细胞毒性/抑制（CD8）的平均比值为1.00（范围0.11 - 3.15）。在未接受照射的肿瘤中，CD随年龄增长而降低，CD4随年龄增长而升高（p < 0.02）。