• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大豆中PLC家族的全基因组分析及GmPI-PLC7对干旱和盐胁迫的响应检测

Genomic-Wide Analysis of the PLC Family and Detection of GmPI-PLC7 Responses to Drought and Salt Stresses in Soybean.

作者信息

Chen Zhi-Feng, Ru Jing-Na, Sun Guo-Zhong, Du Yan, Chen Jun, Zhou Yong-Bin, Chen Ming, Ma You-Zhi, Xu Zhao-Shi, Zhang Xiao-Hong

机构信息

College of Life Sciences, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, China.

Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, China.

出版信息

Front Plant Sci. 2021 Mar 3;12:631470. doi: 10.3389/fpls.2021.631470. eCollection 2021.

DOI:10.3389/fpls.2021.631470
PMID:33763092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7982816/
Abstract

Phospholipase C (PLC) performs significant functions in a variety of biological processes, including plant growth and development. The PLC family of enzymes principally catalyze the hydrolysis of phospholipids in organisms. This exhaustive exploration of soybean GmPLC members using genome databases resulted in the identification of 15 phosphatidylinositol-specific PLC (GmPI-PLC) and 9 phosphatidylcholine-hydrolyzing PLC (GmNPC) genes. Chromosomal location analysis indicated that GmPLC genes mapped to 10 of the 20 soybean chromosomes. Phylogenetic relationship analysis revealed that GmPLC genes distributed into two groups in soybean, the PI-PLC and NPC groups. The expression patterns and tissue expression analysis showed that GmPLCs were differentially expressed in response to abiotic stresses. was selected to further explore the role of PLC in soybean response to drought and salt stresses by a series of experiments. Compared with the transgenic empty vector (EV) control lines, over-expression of (OE) conferred higher drought and salt tolerance in soybean, while the -RNAi (RNAi) lines exhibited the opposite phenotypes. Plant tissue staining and physiological parameters observed from drought- and salt-stressed plants showed that stress increased the contents of chlorophyll, oxygen free radical (O ), hydrogen peroxide (HO) and NADH oxidase (NOX) to amounts higher than those observed in non-stressed plants. This study provides new insights in the functional analysis of genes in response to abiotic stresses.

摘要

磷脂酶C（PLC）在多种生物过程中发挥着重要作用，包括植物的生长和发育。PLC家族的酶主要催化生物体中磷脂的水解。利用基因组数据库对大豆GmPLC成员进行的全面探索，鉴定出了15个磷脂酰肌醇特异性PLC（GmPI-PLC）基因和9个磷脂酰胆碱水解PLC（GmNPC）基因。染色体定位分析表明，GmPLC基因分布在大豆20条染色体中的10条上。系统发育关系分析显示，大豆中的GmPLC基因分为两组，即PI-PLC组和NPC组。表达模式和组织表达分析表明，GmPLCs在响应非生物胁迫时存在差异表达。通过一系列实验，选择了 进一步探究PLC在大豆对干旱和盐胁迫响应中的作用。与转基因空载体（EV）对照系相比， （OE）过表达使大豆具有更高的干旱和盐耐受性，而 -RNAi（RNAi）系则表现出相反的表型。对干旱和盐胁迫处理的植物进行的植物组织染色和生理参数观察表明，胁迫使叶绿素、氧自由基（O ）、过氧化氢（HO）和NADH氧化酶（NOX）的含量增加，高于未胁迫植物中的含量。本研究为 基因在响应非生物胁迫方面的功能分析提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/afe457e06e0a/fpls-12-631470-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/7b1f1678c787/fpls-12-631470-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/f6c3e88af774/fpls-12-631470-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/be599a018af7/fpls-12-631470-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/e75e851dcb25/fpls-12-631470-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/d02376e66cbc/fpls-12-631470-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/0ce15ca1f48b/fpls-12-631470-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/41ffee6f4361/fpls-12-631470-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/f25de225390e/fpls-12-631470-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/b91707805269/fpls-12-631470-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/a94c94175cc4/fpls-12-631470-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/9b04dceb6607/fpls-12-631470-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/908b1751b50b/fpls-12-631470-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/afe457e06e0a/fpls-12-631470-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/7b1f1678c787/fpls-12-631470-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/f6c3e88af774/fpls-12-631470-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/be599a018af7/fpls-12-631470-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/e75e851dcb25/fpls-12-631470-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/d02376e66cbc/fpls-12-631470-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/0ce15ca1f48b/fpls-12-631470-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/41ffee6f4361/fpls-12-631470-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/f25de225390e/fpls-12-631470-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/b91707805269/fpls-12-631470-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/a94c94175cc4/fpls-12-631470-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/9b04dceb6607/fpls-12-631470-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/908b1751b50b/fpls-12-631470-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dbc/7982816/afe457e06e0a/fpls-12-631470-g013.jpg

相似文献

1
Genomic-Wide Analysis of the PLC Family and Detection of GmPI-PLC7 Responses to Drought and Salt Stresses in Soybean.大豆中PLC家族的全基因组分析及GmPI-PLC7对干旱和盐胁迫的响应检测
Front Plant Sci. 2021 Mar 3;12:631470. doi: 10.3389/fpls.2021.631470. eCollection 2021.
2
Genome-Wide Analysis of the DUF4228 Family in Soybean and Functional Identification of in Response to Drought and Salt Stresses.大豆中DUF4228家族的全基因组分析及其对干旱和盐胁迫响应的功能鉴定
Front Plant Sci. 2021 May 17;12:628299. doi: 10.3389/fpls.2021.628299. eCollection 2021.
3
Genome-Wide Analysis of the DYW Subgroup PPR Gene Family and Identification of Responses to Drought Stress.全基因组分析 DYW 亚家族 PPR 基因家族和鉴定对干旱胁迫的响应。
Int J Mol Sci. 2019 Nov 12;20(22):5667. doi: 10.3390/ijms20225667.
4
Genome-wide analysis of the SCAMPs gene family of soybean and functional identification of GmSCAMP5 in salt tolerance.大豆 SCAMPs 基因家族的全基因组分析及 GmSCAMP5 在耐盐性中的功能鉴定。
BMC Plant Biol. 2023 Dec 8;23(1):628. doi: 10.1186/s12870-023-04649-2.
5
Genome-Wide Analysis of the C2 Domain Family in Soybean and Identification of a Putative Abiotic Stress Response Gene .大豆中C2结构域家族的全基因组分析及一个假定的非生物胁迫响应基因的鉴定
Front Plant Sci. 2021 Feb 16;12:620544. doi: 10.3389/fpls.2021.620544. eCollection 2021.
6
Comprehensive genomic analysis and expression profiling of phospholipase C gene family during abiotic stresses and development in rice.在非生物胁迫和水稻发育过程中对 PLC 基因家族进行全基因组分析和表达谱分析。
PLoS One. 2013 Apr 30;8(4):e62494. doi: 10.1371/journal.pone.0062494. Print 2013.
7
Genome-Wide Analysis and Expression Profiling of the Phospholipase C Gene Family in Soybean (Glycine max).大豆（Glycine max）中磷脂酶C基因家族的全基因组分析及表达谱分析
PLoS One. 2015 Sep 30;10(9):e0138467. doi: 10.1371/journal.pone.0138467. eCollection 2015.
8
Functional Analysis of the Soybean Gene Responding to Drought and Salt Stresses.干旱和盐胁迫响应大豆基因的功能分析。
Int J Mol Sci. 2019 Nov 25;20(23):5909. doi: 10.3390/ijms20235909.
9
Role for Arabidopsis in Stomatal Movement, Seed Mucilage Attachment, and Leaf Serration.拟南芥在气孔运动、种子黏液附着和叶片锯齿形成中的作用。
Front Plant Sci. 2018 Nov 27;9:1721. doi: 10.3389/fpls.2018.01721. eCollection 2018.
10
Genome-Wide Characterization and Expression Analysis of Soybean TGA Transcription Factors Identified a Novel TGA Gene Involved in Drought and Salt Tolerance.大豆TGA转录因子的全基因组鉴定与表达分析鉴定出一个参与耐旱和耐盐的新TGA基因。
Front Plant Sci. 2019 May 16;10:549. doi: 10.3389/fpls.2019.00549. eCollection 2019.

引用本文的文献

1
Plant PI-PLC signaling in stress and development.植物磷脂酰肌醇特异性磷脂酶C信号传导在应激和发育过程中的作用
Plant Physiol. 2025 Feb 7;197(2). doi: 10.1093/plphys/kiae534.
2
Genome-wide association study reveals genetic basis and candidate genes for chlorophyll content of leaves in maize (Z L.).全基因组关联研究揭示了玉米叶片叶绿素含量的遗传基础和候选基因。
PeerJ. 2024 Oct 7;12:e18278. doi: 10.7717/peerj.18278. eCollection 2024.
3
Exploring the Role of in Heat Tolerance at Seedling and Adult Stages of Wheat through Transcriptome Analysis.

本文引用的文献

1
Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms.拟南芥磷脂酶C亚型的基因特异性表达与钙激活
New Phytol. 2004 Jun;162(3):643-654. doi: 10.1111/j.1469-8137.2004.01069.x.
2
Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean.大豆中GDSL型酯酶/脂肪酶基因家族的全基因组鉴定、进化及表达
Front Plant Sci. 2020 Jun 25;11:726. doi: 10.3389/fpls.2020.00726. eCollection 2020.
3
Non-specific phospholipase C (NPC): an emerging class of phospholipase C in plant growth and development.
通过转录组分析探索在小麦幼苗和成株期耐热性中的作用。
Int J Mol Sci. 2023 Nov 21;24(23):16583. doi: 10.3390/ijms242316583.
4
Ectopic Expression of Distinct PLC Genes Identifies 'Compactness' as a Possible Architectural Shoot Strategy to Cope with Drought Stress.不同 PLC 基因的异位表达鉴定出“紧凑性”可能是一种应对干旱胁迫的建筑性 Shoot 策略。
Plant Cell Physiol. 2024 Jun 27;65(6):885-903. doi: 10.1093/pcp/pcad123.
5
Unfolding molecular switches for salt stress resilience in soybean: recent advances and prospects for salt-tolerant smart plant production.大豆中用于耐盐性的分子开关展开：耐盐智能植物生产的最新进展与展望
Front Plant Sci. 2023 Apr 19;14:1162014. doi: 10.3389/fpls.2023.1162014. eCollection 2023.
6
Grapevine-Associated Lipid Signalling Is Specifically Activated in an Background in Response to an Aggressive Pathovar.葡萄蔓关联脂质信号在对侵袭性致病变种的响应中特异性地在 背景中被激活。
Cells. 2023 Jan 21;12(3):394. doi: 10.3390/cells12030394.
7
Comprehensive physiological, transcriptomic, and metabolomic analyses reveal the synergistic mechanism of G5 combined with silicon alleviate oxidative stress in drought-stressed Fisch.综合生理、转录组和代谢组分析揭示了G5与硅结合减轻干旱胁迫下费氏弧菌氧化应激的协同机制。
Front Plant Sci. 2022 Dec 8;13:1033915. doi: 10.3389/fpls.2022.1033915. eCollection 2022.
8
Progress in Soybean Genetic Transformation Over the Last Decade.过去十年大豆遗传转化的进展
Front Plant Sci. 2022 Jun 9;13:900318. doi: 10.3389/fpls.2022.900318. eCollection 2022.
9
Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the Gene in Abiotic Stress Responses.大豆中类Tubby蛋白的综合分析及其基因在非生物胁迫响应中的作用
Front Plant Sci. 2022 Apr 25;13:844545. doi: 10.3389/fpls.2022.844545. eCollection 2022.
10
Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations.植物激素信号中的磷脂酸：从靶蛋白到膜构象。
Int J Mol Sci. 2022 Mar 17;23(6):3227. doi: 10.3390/ijms23063227.
非特异性磷脂酶 C（NPC）：植物生长发育中新兴的一类磷脂酶 C。
J Plant Res. 2020 Jul;133(4):489-497. doi: 10.1007/s10265-020-01199-8. Epub 2020 May 5.
4
Identification and characterization of GmMYB118 responses to drought and salt stress.鉴定和分析 GmMYB118 对干旱和盐胁迫的响应。
BMC Plant Biol. 2018 Dec 3;18(1):320. doi: 10.1186/s12870-018-1551-7.
5
Arabidopsis Phospholipase C3 is Involved in Lateral Root Initiation and ABA Responses in Seed Germination and Stomatal Closure.拟南芥磷脂酶 C3 参与侧根起始和 ABA 响应在种子萌发和气孔关闭。
Plant Cell Physiol. 2018 Mar 1;59(3):469-486. doi: 10.1093/pcp/pcx194.
6
Drought Tolerance Conferred in Soybean (Glycine max. L) by GmMYB84, a Novel R2R3-MYB Transcription Factor.GmMYB84，一种新型 R2R3-MYB 转录因子，赋予大豆（Glycine max. L）耐旱性。
Plant Cell Physiol. 2017 Oct 1;58(10):1764-1776. doi: 10.1093/pcp/pcx111.
7
A phosphoinositide-specific phospholipase C pathway elicits stress-induced Ca signals and confers salt tolerance to rice.一个磷酯酰肌醇特异性磷脂酶 C 途径引发胁迫诱导的 Ca 信号，并赋予水稻耐盐性。
New Phytol. 2017 May;214(3):1172-1187. doi: 10.1111/nph.14426. Epub 2017 Feb 3.
8
Molecular Soybean-Pathogen Interactions.分子大豆-病原体相互作用。
Annu Rev Phytopathol. 2016 Aug 4;54:443-68. doi: 10.1146/annurev-phyto-080615-100156. Epub 2016 Jan 17.
9
Plant phospholipases D and C and their diverse functions in stress responses.植物磷脂酶 D 和 C 及其在应激反应中的多种功能。
Prog Lipid Res. 2016 Apr;62:55-74. doi: 10.1016/j.plipres.2016.01.002. Epub 2016 Jan 16.
10
Lipid signalling in plant responses to abiotic stress.植物对非生物胁迫响应中的脂质信号传导
Plant Cell Environ. 2016 May;39(5):1029-48. doi: 10.1111/pce.12666. Epub 2016 Feb 12.