人副流感病毒4型感染：1998年至2002年20例报告
Human parainfluenza virus type 4 infections: a report of 20 cases from 1998 to 2002.
作者信息
Billaud Geneviève, Morfin Florence, Vabret Astrid, Boucher Anne, Gillet Yves, Crassard Nicolas, Galambrun Claire, Ferraris Olivier, Legrand Loïc, Aymard Michèle, Lina Bruno, Freymuth François, Thouvenot Danielle
机构信息
Laboratoire de Virologie, Domaine Rockefeller, Hospices Civils de Lyon, 69373 Lyon Cedex 08, Lyon, France.
出版信息
J Clin Virol. 2005 Sep;34(1):48-51. doi: 10.1016/j.jcv.2005.02.001.
BACKGROUND
Human Parainfluenza Viruses (HPIV) type 4 are responsible for respiratory infections. Unlike HPIV types 1-3, they are associated with mild infections and appear to be infrequent. Thus, they often go undetected.
STUDY DESIGN
From 1998 to 2002, in 20 respiratory samples of hospitalised patient, we isolated viruses presenting a large syncytial cytopathic effect when inoculated on LLC-MK2 cells. Most of the patients (16/20) were young infants and all of them presented with respiratory infections.
RESULTS
We detected 18 cases during autumn and winter, 1 case during spring and 1 during summer. We could not identify these viruses using the panel of routine assays. Samples were then analysed by specific HPIV 4 RT-PCR and IF assays. All the samples were scored positive with both methods.
CONCLUSION
We conclude that HPIV 4 infections are probably underestimated. Their role in viral respiratory infections should be carefully investigated using techniques adapted to their detection and culture.
背景
4型人副流感病毒（HPIV）可引发呼吸道感染。与1 - 3型HPIV不同，它们引起的感染症状较轻，且似乎较为罕见。因此，它们常常未被检测到。
研究设计
1998年至2002年期间，在20份住院患者的呼吸道样本中，我们分离出了接种于LLC - MK2细胞时呈现出明显合胞体细胞病变效应的病毒。大多数患者（16/20）为幼儿，且均患有呼吸道感染。
结果
我们在秋季和冬季检测到18例，春季检测到1例，夏季检测到1例。使用常规检测方法我们无法鉴定这些病毒。随后通过特异性HPIV 4逆转录聚合酶链反应（RT - PCR）和免疫荧光（IF）检测对样本进行分析。两种方法检测所有样本均呈阳性。
结论
我们得出结论，HPIV 4感染可能被低估了。应使用适合其检测和培养的技术仔细研究它们在病毒性呼吸道感染中的作用。
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