Hattoufi Kenza, Tligui Houssain, Obtel Majdouline, El Ftouh Sobha, Kharbach Aicha, Barkat Amina
National Reference Center in Neonatology and Nutrition, Children's Hospital, University Hospital Centre IBN SINA, Rabat, Morocco.
Research Team on Health and Nutrition of Mother and Child, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco.
Adv Virol. 2020 Feb 18;2020:6212643. doi: 10.1155/2020/6212643. eCollection 2020.
In Morocco, pediatric pneumonia remains a serious public health problem, as it constitutes the first cause of mortality due to infectious diseases. The etiological diagnosis of acute respiratory tract infections is difficult. Therefore, it is necessary to use Multiplex real-time polymerase chain reaction assay tests in a routine setting for exact and fast identification.
In this paper, we present the clinical results of pediatric pneumonia and describe their etiology by using molecular diagnosis. : Tracheal secretion was collected from infants presenting respiratory distress isolated or associated with systemic signs, attending the unit of Neonatology between December 1, 2016, and Mai 31, 2018. Samples were tested with the multiplex RespiFinder® SMART 22 FAST which potentially detects 18 viruses and 4 bacteria.
Of the 86 infants considered in this study (mean age 31 ± 19 days) suspected of acute respiratory tract infections, 71 (83%) were positive for one or multiple viruses or/and bacteria. The majority of acute respiratory tract infections had a viral origin (95%): respiratory syncytial viruses (A and B) (49%), rhinovirus (21%), coronaviruses 229E (11%), humain metapneumovirus (5%), influenza A (3%), influenza H1N1 (1%), adenovirus (2%), and parainfluenza virus type 4 (2%). Among our patients, 6% had . Coinfections were not associated with severe respiratory symptoms.
The clinical spectrum of respiratory infections is complex and often nonspecific. Thus, the early and fast detection of related causative agents is crucial. The use of multiplex real time polymerase chain reaction may help choose an accurate treatment, reduce the overall use of unnecessary antibiotics, preserve intestinal flora, and decrease nosocomial infection by reducing the length of hospitalization.
在摩洛哥，小儿肺炎仍然是一个严重的公共卫生问题，它是传染病导致死亡的首要原因。急性呼吸道感染的病因诊断困难。因此，有必要在常规检查中使用多重实时聚合酶链反应检测来进行准确快速的鉴定。
在本文中，我们展示了小儿肺炎的临床结果，并通过分子诊断描述其病因。从2016年12月1日至2018年5月31日在新生儿科就诊、出现孤立性呼吸窘迫或伴有全身症状的婴儿中收集气管分泌物。样本使用多重RespiFinder® SMART 22 FAST进行检测，该检测可潜在检测18种病毒和4种细菌。
在本研究中考虑的86例疑似急性呼吸道感染的婴儿（平均年龄31±19天）中，71例（83%）一种或多种病毒和/或细菌检测呈阳性。大多数急性呼吸道感染由病毒引起（95%）：呼吸道合胞病毒（A和B）（49%）、鼻病毒（21%）、冠状病毒229E（11%）、人偏肺病毒（5%）、甲型流感病毒（3%）、甲型H1N1流感病毒（1%）、腺病毒（2%）和4型副流感病毒（2%）。在我们的患者中，6%有……合并感染与严重呼吸道症状无关。
呼吸道感染的临床谱复杂且通常无特异性。因此，早期快速检测相关病原体至关重要。使用多重实时聚合酶链反应可能有助于选择准确的治疗方法，减少不必要抗生素的总体使用，保护肠道菌群，并通过缩短住院时间减少医院感染。
