Mendes-Giannini Maria José Soares, Soares Christiane Pienna, da Silva Juliana Leal Monteiro, Andreotti Patrícia Ferrari
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, Sao Paulo, Brazil.
FEMS Immunol Med Microbiol. 2005 Sep 1;45(3):383-94. doi: 10.1016/j.femsim.2005.05.014.
This review provides an overview of several molecular and cellular approaches that are likely to supply insights into the host-fungus interaction. Fungi present intra- and/or extracellular host-parasite interfaces, the parasitism phenomenon being dependent on complementary surface molecules. The entry of the pathogen into the host cell is initiated by the fungus adhering to the cell surface, which generates an uptake signal that may induce its cytoplasmatic internalization. Furthermore, microbial pathogens use a variety of their surface molecules to bind to host extracellular matrix (ECM) components to establish an effective infection. On the other hand, integrins mediate the tight adhesion of cells to the ECM at sites referred to as focal adhesions and also play a role in cell signaling. The phosphorylation process is an important mechanism of cell signaling and regulation; it has been implicated recently in defense strategies against a variety of pathogens that alter host-signaling pathways in order to facilitate their invasion and survival within host cells. The study of signal transduction pathways in virulent fungi is especially important in view of their putative role in the regulation of pathogenicity. This review discusses fungal adherence, changes in cytoskeletal organization and signal transduction in relation to host-fungus interaction.
本综述概述了几种可能为宿主 - 真菌相互作用提供见解的分子和细胞方法。真菌呈现细胞内和/或细胞外的宿主 - 寄生虫界面，寄生现象依赖于互补的表面分子。病原体进入宿主细胞是由真菌粘附到细胞表面引发的，这会产生一个摄取信号，可能诱导其细胞质内化。此外，微生物病原体利用其多种表面分子与宿主细胞外基质（ECM）成分结合，以建立有效的感染。另一方面，整合素介导细胞在称为粘着斑的部位与ECM紧密粘附，并且在细胞信号传导中也起作用。磷酸化过程是细胞信号传导和调节的重要机制；最近它与针对多种病原体的防御策略有关，这些病原体改变宿主信号通路以促进其在宿主细胞内的侵袭和存活。鉴于其在致病性调节中的假定作用，对致病真菌中信号转导途径的研究尤为重要。本综述讨论了与宿主 - 真菌相互作用相关的真菌粘附、细胞骨架组织变化和信号转导。
