Seiliev A A, Zvonareva N B, Zhivotovsky B D, Hanson K P
Central Research Institute of Roentgenology and Radiology, Russian Ministry of Public Health, St. Petersburg.
Radiat Environ Biophys. 1992;31(2):123-32. doi: 10.1007/BF01211210.
The spectrum of nuclear nucleases in control and irradiated (4 Gy) thymocytes has been investigated. Using the method of SDS electrophoresis of nuclear proteins in 3H-DNA-polyacrylamide gels a number of polypeptides of MW. 35, 32, 17.7, 17.2 and 16.4 kDa possessing nuclease activity were found. The 35 kDa enzyme is only active in the presence of Ca2+ and Mg2+ ions. In response to cycloheximide injection (3 mg/100 g body weight) and irradiation, we did not detect the 35 kDa nuclease activity. Nucleases of 32, 17.7, 17.2 and 16.4 kDa are active in the presence of Ca2+ ions. The activities of these nucleases increases 60 min after irradiation. These nucleases were also found in the fraction of polydeoxyribonucleotide (PDN).
对对照及经4 Gy照射的胸腺细胞中的核酸酶谱进行了研究。采用3H-DNA-聚丙烯酰胺凝胶中核蛋白的SDS电泳方法，发现了一些具有核酸酶活性、分子量分别为35、32、17.7、17.2和16.4 kDa的多肽。35 kDa的酶仅在Ca2+和Mg2+离子存在时具有活性。注射环己酰亚胺（3 mg/100 g体重）并照射后，未检测到35 kDa核酸酶的活性。32、17.7、17.2和16.4 kDa的核酸酶在Ca2+离子存在时具有活性。照射60分钟后，这些核酸酶的活性增加。在多脱氧核糖核苷酸（PDN）组分中也发现了这些核酸酶。
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