Seĭliev A A, Khanson K P
Biull Eksp Biol Med. 1990 Nov;110(11):484-6.
Spectra of thymocyte's nuclear nucleases of control and glucocorticoid treated (5 mg/kg body weight) adrenalectomized rats have been investigated. Using the method of SDS-electrophoresis of nuclear proteins in 3H-DNA-polyacrylamide gel (PAAG) the authors managed to discover a number of polypeptides of 35, 32, 17.7, 17.0, 16.4 kDa molecular mass possessing a nuclease activity. The enzyme of 35 kDa is only active in the presence of Ca2+ and Mg2+ ions and inhibited by cycloheximide. Nucleases of 32, 17.7, 17.0, 16.4 kDa are active in the presence of Ca2+ ions. The enzymic activity of these nucleases increases 60 min after steroid treatment. Nuclease of 17.7, 17.0, 16.4 kDa are poly(ADP-ribosylated). Glucocorticoid mediated activation don't blocked by poly(ADP-ribosylation). Possible role and mechanism of discovered nucleases are discussed.
对对照组和经糖皮质激素（5毫克/千克体重）处理的去肾上腺大鼠胸腺细胞核核酸酶的光谱进行了研究。作者采用3H-DNA-聚丙烯酰胺凝胶（PAAG）中核蛋白的SDS电泳方法，成功发现了一些分子量分别为35、32、17.7、17.0、16.4千道尔顿且具有核酸酶活性的多肽。35千道尔顿的酶仅在Ca2+和Mg2+离子存在时具有活性，并受环己酰亚胺抑制。32、17.7、17.0、16.4千道尔顿的核酸酶在Ca2+离子存在时具有活性。这些核酸酶的酶活性在类固醇处理后60分钟增加。17.7、17.0、16.4千道尔顿的核酸酶是多聚（ADP-核糖基化）的。糖皮质激素介导的激活不受多聚（ADP-核糖基化）的阻断。文中讨论了所发现核酸酶的可能作用和机制。
