Aleksandrova S E, Ivannik B P, Gulaev V A, Riabchenko N I
Radiobiologiia. 1988 Mar-Apr;28(2):213-8.
The level of chromatin degradation was studied and the method of electron-microscopy was used to estimate the changes in the ultrastructure of irradiated and nonirradiated thymocytes of rats treated with cycloheximide. The latter was found to decrease the degree of derangement of nuclear ultrastructure and the level of chromatin degradation in exposed animals and to increase the yield of these damages in thymocytes of nonirradiated animals. The electronmicroscopic determinations showed that the percentage of thymocytes with the impaired nucleus structure is twice as high as that of degraded chromatin. The causes of the quantitative disagreement between the morphological and biochemical indices of the interphase thymocyte death are discussed.
研究了染色质降解水平，并采用电子显微镜方法评估用环己酰亚胺处理的大鼠受辐照和未受辐照胸腺细胞超微结构的变化。结果发现，环己酰亚胺可降低受照动物的核超微结构紊乱程度和染色质降解水平，并增加未受辐照动物胸腺细胞中这些损伤的发生率。电子显微镜测定表明，核结构受损的胸腺细胞百分比是染色质降解细胞的两倍。文中讨论了间期胸腺细胞死亡的形态学和生化指标定量不一致的原因。
