Xie Changan, Chen De, Li Yong-Qing
Department of Physics, East Carolina University, Greenville, North Carolina 27858-4353, USA.
Opt Lett. 2005 Jul 15;30(14):1800-2. doi: 10.1364/ol.30.001800.
We report on a novel technique for sorting and identification of single biological cells and food-borne bacteria based on laser tweezers and Raman spectroscopy (LTRS). With this technique, biological cells of different physiological states in a sample chamber were identified by their Raman spectral signatures and then they were selectively manipulated into a clean collection chamber with optical tweezers through a microchannel. As an example, we sorted the live and dead yeast cells into the collection chamber and validated this with a standard staining technique. We also demonstrated that bacteria existing in spoiled foods could be discriminated from a variety of food particles based on their characteristic Raman spectra and then isolated with laser manipulation. This label-free LTRS sorting technique may find broad applications in microbiology and rapid examination of food-borne diseases.
我们报道了一种基于激光镊子和拉曼光谱（LTRS）的用于单个生物细胞和食源细菌分类与鉴定的新技术。利用该技术，通过拉曼光谱特征识别样品腔中不同生理状态的生物细胞，然后用光学镊子通过微通道将它们选择性地操控到一个干净的收集腔中。例如，我们将活酵母细胞和死酵母细胞分类到收集腔中，并用标准染色技术进行了验证。我们还证明，基于其特征拉曼光谱，可以从各种食物颗粒中区分出变质食物中存在的细菌，然后用激光操控将其分离。这种无标记的LTRS分类技术可能在微生物学和食源性疾病的快速检测中得到广泛应用。
