Department of Chemical Engineering, Virginia Tech, Blacksburg, VA 24061, USA.
Analyst. 2017 Dec 18;143(1):60-80. doi: 10.1039/c7an01346a.
The inherent heterogeneity in cell populations has become of great interest and importance as analytical techniques have improved over the past decades. With the advent of personalized medicine, understanding the impact of this heterogeneity has become an important challenge for the research community. Many different microfluidic approaches with varying levels of throughput and resolution exist to study single cell activity. In this review, we take a broad view of the recent microfluidic developments in single cell analysis based on microwell, microchamber, and droplet platforms. We cover physical, chemical, and molecular biology approaches for cellular and molecular analysis including newly emerging genome-wide analysis.
细胞群体的固有异质性已经成为一个非常有趣和重要的研究领域，因为在过去几十年中，分析技术得到了改进。随着个性化医疗的出现，研究界已经认识到了解这种异质性的影响是一个重要的挑战。为了研究单细胞活性，已经有许多不同的微流控方法，其通量和分辨率各不相同。在这篇综述中，我们广泛介绍了基于微井、微腔和液滴平台的单细胞分析的最新微流控技术进展。我们涵盖了用于细胞和分子分析的物理、化学和分子生物学方法，包括新出现的全基因组分析。
