Aouacheria Abdel, Brunet Frédéric, Gouy Manolo
Laboratoire de Biométrie et Biologie Evolutive, Université Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France.
Mol Biol Evol. 2005 Dec;22(12):2395-416. doi: 10.1093/molbev/msi234. Epub 2005 Aug 10.
In this report, we conducted a comprehensive survey of Bcl-2 family members, a divergent group of proteins that regulate programmed cell death by an evolutionarily conserved mechanism. Using comparative sequence analysis, we found novel sequences in mammals, nonmammalian vertebrates, and in a number of invertebrates. We then asked what conclusions could be drawn from phyletic distribution, intron/exon structures, sequence/structure relationships, and phylogenetic analyses within the updated Bcl-2 family. First, multidomain members having a sequence pattern consistent with the conservation of the Bcl-X(L)/Bax/Bid topology appear to be restricted to multicellular animals and may share a common ancestry. Next, BNip proteins, which were originally identified based on their ability to bind to E1B 19K/Bcl-2 proteins, form three independent monophyletic branches with different evolutionary history. Lastly, a set of Bcl-2 homology 3-only proteins with unrelated secondary structures seems to have evolved after the origin of Metazoa and exhibits diverse expansion after speciation during vertebrate evolution.
在本报告中，我们对Bcl-2家族成员进行了全面调查，这是一组通过进化保守机制调节程序性细胞死亡的不同蛋白质。通过比较序列分析，我们在哺乳动物、非哺乳动物脊椎动物和一些无脊椎动物中发现了新序列。然后，我们探讨了从更新后的Bcl-2家族的系统发育分布、内含子/外显子结构、序列/结构关系和系统发育分析中可以得出哪些结论。首先，具有与Bcl-X(L)/Bax/Bid拓扑结构保守一致的序列模式的多结构域成员似乎仅限于多细胞动物，并且可能有共同的祖先。其次，最初根据其与E1B 19K/Bcl-2蛋白结合的能力鉴定出的BNip蛋白形成了三个具有不同进化历史的独立单系分支。最后，一组具有不相关二级结构的仅含Bcl-2同源结构域3的蛋白似乎在后生动物起源后进化而来，并在脊椎动物进化过程中的物种形成后表现出多样化的扩张。
