暴露于1,1-二甲基肼后对小鼠淋巴细胞膜电位、细胞内游离钙和白细胞介素-2受体表达的评估。

Evaluation of murine lymphocyte membrane potential, intracellular free calcium, and interleukin-2 receptor expression upon exposure to 1,1-dimethylhydrazine.

作者信息

Frazier D E, Tarr M J, Olsen R G

机构信息

Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.

出版信息

Toxicol Lett. 1992 Jun;61(1):27-37. doi: 10.1016/0378-4274(92)90060-w.

DOI:10.1016/0378-4274(92)90060-w

PMID:1609436

Abstract

The effects of 1,1-dimethylhydrazine (UDMH) on several early events associated with lymphocyte activation were examined. The concentration of intracellular calcium ([Ca2+]i) and membrane potential of murine lymphocytes were found to be altered upon exposure to UDMH; [Ca2+]i was increased in murine thymocytes, while splenocytes exhibited membrane hyperpolarization. In addition, interleukin-2 receptor expression induced by in-vitro concanavalin A stimulation of murine splenocytes at 24 and 48 h in the presence of UDMH was not affected. UDMH may interfere with the ability of these two distinct lymphocyte populations to regulate normal ionic fluctuations, thus contributing to altered immune responsiveness.

摘要

研究了偏二甲肼（UDMH）对与淋巴细胞激活相关的几个早期事件的影响。发现暴露于UDMH后，小鼠淋巴细胞的细胞内钙浓度（[Ca2+]i）和膜电位发生了改变；小鼠胸腺细胞中的[Ca2+]i增加，而脾细胞则表现出膜超极化。此外，在UDMH存在的情况下，体外伴刀豆球蛋白A刺激小鼠脾细胞24小时和48小时诱导的白细胞介素-2受体表达未受影响。UDMH可能会干扰这两种不同淋巴细胞群体调节正常离子波动的能力，从而导致免疫反应性改变。