Jenski L J, Scherer J M, Caldwell L D, Ney V A, Stillwell W
Department of Biology, Indiana University-Purdue University at Indianapolis 46202-5132, USA.
Lipids. 1998 Sep;33(9):869-78. doi: 10.1007/s11745-998-0283-x.
Docosahexaenoic acid (DHA) is an n-3 fatty acid beneficial to several human conditions including inflammation and autoimmune disease. To better understand the effect of DHA on immunity, we monitored the rise in cytosolic free calcium, interleukin 2 receptor (IL2R) expression, and proliferation of splenic lymphocytes triggered with three different stimuli in the presence or absence of DHA. We found that 10 microg DHA/mL suppressed concanavalin A-induced mitogenesis and the mixed lymphocyte reaction while concurrently enhancing proliferation stimulated with anti-Thy-1 antibodies. Proliferation, as measured by [3H]thymidine incorporation after 2 to 5 d of culture, was affected by DHA, but earlier activation effects such as elevation of cytosolic free calcium and IL2R expression were not altered. These results imply that DHA incorporated into membrane phospholipids differentially affects the activity of distinct membrane-bound receptors and signaling molecules. This result suggests that DHA may be used to modulate immune responses selectively, e.g., to suppress undesired autoimmunity while maintaining protective immunity.
二十二碳六烯酸（DHA）是一种对包括炎症和自身免疫性疾病在内的多种人类疾病有益的n-3脂肪酸。为了更好地理解DHA对免疫的影响，我们监测了在有或没有DHA存在的情况下，三种不同刺激引发的脾淋巴细胞胞质游离钙升高、白细胞介素2受体（IL2R）表达和增殖情况。我们发现，10微克DHA/毫升可抑制伴刀豆球蛋白A诱导的有丝分裂和混合淋巴细胞反应，同时增强抗Thy-1抗体刺激的增殖。培养2至5天后通过[3H]胸苷掺入法测量的增殖受DHA影响，但早期激活效应如胞质游离钙升高和IL2R表达未改变。这些结果表明，掺入膜磷脂中的DHA对不同膜结合受体和信号分子的活性有不同影响。这一结果表明，DHA可用于选择性调节免疫反应，例如，在维持保护性免疫的同时抑制不必要的自身免疫。
