Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.
Division of Life Sciences, Institute of Advanced Study in Science and Technology, Vigyan Path, Garchuk, Paschim Boragaon, Guwahati 781035, Assam, India.
Toxins (Basel). 2023 Aug 14;15(8):504. doi: 10.3390/toxins15080504.
Indian Red Scorpion () stings are a neglected public health problem in tropical and sub-tropical countries, including India. The drawbacks of conventional therapies using commercial anti-scorpion antivenom (ASA) and α1-adrenoreceptor antagonists (AAA) have prompted us to search for an adequate formulation to improve treatment against stings. Novel therapeutic drug formulations (TDF) of low doses of commercial ASA, AAA, and ascorbic acid have remarkably improved in neutralising the in vivo toxic effects of venom (MTV) tested in and Wistar strain albino rats in vivo models. The neutralisation of MTV-induced production of free radicals, alteration of the mitochondrial transmembrane potential, and upregulated expression of genes involved in apoptosis, detoxification, and stress response in by TDF surpassed the same effect shown by individual components of the TDF. Further, TDF efficiently neutralized the MTV-induced increase in blood glucose level within 30 to 60 min post-treatment, organ tissue damage, necrosis, and pulmonary oedema in Wistar rats, indicating its clinical application for effecting treating envenomation. This study demonstrates for the first time that can be a model organism for screening the neutralization potency of the drug molecules against a neurotoxic scorpion venom.
印度红蝎子 () 蜇伤是热带和亚热带国家（包括印度）未被重视的公共卫生问题。由于传统的使用商业抗蝎毒液（ASA）和α1-肾上腺素受体拮抗剂（AAA）的治疗方法存在缺陷，我们促使我们寻找一种合适的制剂来改善对 蜇伤的治疗效果。新型治疗药物制剂（TDF）中低剂量的商业 ASA、AAA 和抗坏血酸，在体内模型中显著改善了中和 毒液（MTV）的体内毒性作用，在 和 Wistar 白化大鼠中进行了测试。TDF 对 MTV 诱导的自由基产生、线粒体跨膜电位改变以及参与凋亡、解毒和应激反应的基因表达的上调的中和作用超过了 TDF 单个成分所表现出的相同作用。此外，TDF 还能有效中和 MTV 诱导的 Wistar 大鼠血糖水平在治疗后 30 至 60 分钟内升高、器官组织损伤、坏死和肺水肿，表明其在临床上可用于治疗 中毒。这项研究首次表明， 可以成为筛选针对神经毒性蝎毒液的药物分子中和效力的模式生物。
