Salaj J, Petrovská B, Obert B, Pret'ová A
Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39 A, SK-950 07 Nitra 1, Slovak Republic.
Plant Cell Rep. 2005 Dec;24(10):590-5. doi: 10.1007/s00299-005-0029-x. Epub 2005 Nov 16.
Cultivation of flax hypocotyl segments on MS medium supplemented with auxin (2,4-D, NAA) and combination of auxin (NAA) and cytokinin (BAP, zeatin) resulted in production of callus on the cut ends of segments and prolonged cultivation in globular structures resembling early stages of somatic embryos. Embryo-like structures protruded on the surface directly from the subepidermal layers of hypocotyl segments. Despite these globular structures closely resembling somatic embryos, histological observations did not reveal their embryogenic character-organogenesis was the predominant developmental morphogenic pathway. Based on our experiments, as well as on critical revision of existing reports on flax somatic embryogenesis, we conclude, that there has not yet been convincing histological proof of somatic embyogenesis from flax hypocotyl segments.
在添加生长素（2,4 - D、萘乙酸）以及生长素（萘乙酸）与细胞分裂素（苄氨基嘌呤、玉米素）组合的MS培养基上培养亚麻下胚轴切段，切段的切口端会产生愈伤组织，并且在类似体细胞胚早期阶段的球形结构中进行长时间培养。胚状结构直接从下胚轴切段的表皮下层突出到表面。尽管这些球形结构与体细胞胚非常相似，但组织学观察并未揭示它们的胚性特征——器官发生是主要的发育形态发生途径。基于我们的实验以及对现有亚麻体细胞胚发生报告的批判性审视，我们得出结论，尚未有令人信服的组织学证据证明亚麻下胚轴切段能发生体细胞胚发生。
