Haensch K-T
Department Plant Propagation, Institute of Vegetable and Ornamental Crops Grossbeeren/Erfurt e.V., Kühnhäuser Str. 101, 99189, Erfurt-Kühnhausen, Germany.
Plant Cell Rep. 2004 Jan;22(6):376-81. doi: 10.1007/s00299-003-0726-2. Epub 2003 Oct 21.
Somatic embryo-like structures were produced from the hypocotyls of ten cultivars of Pelargonium x hortorum using the protocols of Marsolais et al. (1991; Can J Bot 69:1188-1193) and Slimmon et al. (1991; Plant Cell Rep 10:587-589) and their embryonic natures evaluated. Nine cultivars responded, and 937 structures were formed. Regeneration corresponded well with published data. The somatic embryo-like structures were globular- to leaf-shaped or similar to shoots. A root pole was never visible. Histological examinations confirmed the lack of bipolarity and revealed vascular connections to the explant in the more developed structures. Therefore, these structures cannot be classified as somatic embryos. The importance of these results is discussed in terms of evaluating published protocols for the propagation of these pelargoniums by somatic embryogenesis from hypocotyls.
采用Marsolais等人（1991年；《加拿大植物学杂志》69:1188 - 1193）以及Slimmon等人（1991年；《植物细胞报告》10:587 - 589）的方法，从十个天竺葵栽培品种的下胚轴中诱导出了体细胞胚样结构，并对其胚胎性质进行了评估。九个品种产生了响应，共形成了937个结构。再生情况与已发表的数据吻合良好。体细胞胚样结构呈球形至叶形或类似芽状，从未观察到根极。组织学检查证实其缺乏双极性，并显示在较成熟的结构中与外植体存在维管连接。因此，这些结构不能归类为体细胞胚。本文从评估已发表的通过下胚轴体细胞胚胎发生繁殖这些天竺葵的方案的角度，讨论了这些结果的重要性。
