Mazza G, Fortunato A, Ferrari E, Canosi U, Falaschi A, Polsinelli M
Istituto di Genetica, Università di Pavia, Italy.
Mol Gen Genet. 1975;136(1):9-30. doi: 10.1007/BF00275445.
We have isolated recombination deficient mutants of Bacillus subtilis on the basis of their sensitivity to methyl-methane-sulfonate or ultraviolet light, or of their inability to be transformed on solid medium. We have analyzed the mutants for several recombination and repair properties; we have grouped them in 5 classes on the basis of their phenotype and tested them for the activity of several enzymes acting on DNA, ie. DNA polymerase, polynucleotide ligase, ATP dependent DNase, and a DNase acting on single-stranded DNA. One mutant was found reduced in the latter DNase. Some of the mutants have been mapped, and they correspond to three different genes denominated rec D, rec F and rec G. All the recombination deficient mutants of B. subtilis described in the literature have been grouped in 7 classes; the mutations belong to 13 (and possibly 15) different genes distributed along the map. A coherent nomenclature and the criteria for a standard study of the rec mutants are proposed.
我们基于枯草芽孢杆菌对甲磺酸甲酯或紫外线的敏感性，或其在固体培养基上无法被转化的特性，分离出了重组缺陷型突变体。我们分析了这些突变体的几种重组和修复特性；根据它们的表型将其分为5类，并检测了它们对几种作用于DNA的酶的活性，即DNA聚合酶、多核苷酸连接酶、依赖ATP的DNA酶和作用于单链DNA的DNA酶。发现有一个突变体的后一种DNA酶活性降低。其中一些突变体已被定位，它们对应于三个不同的基因，分别命名为rec D、rec F和rec G。文献中描述的枯草芽孢杆菌所有重组缺陷型突变体已被分为7类；这些突变属于沿图谱分布的13个（可能15个）不同基因。本文提出了一个连贯的命名法以及rec突变体标准研究的标准。
