de Vos W M, Venema G
Mol Gen Genet. 1982;187(3):439-45. doi: 10.1007/BF00332625.
With the use of two-dimensional gel electrophoresis, the proteins present in a transformation-proficient B. subtilis strain were compared with those present in an isogenic, recombination-deficient strain carrying the recE4 mutation. One protein (molecular weight 45 kD, iso-electric point 5.4) was found to be virtually absent in the recE4 strain. This 45 kD protein is a prominent protein predominantly present in the competent fraction of a competent culture. The synthesis of the protein is substantially stimulated by irradiation with ultraviolet light or treatment with mitomycin C and, to a lesser extent, by treatment with nalidixic acid. Since the protein is also observed in a strain cured for SP beta and carrying non-inducible PBS X, it is unlikely that this protein is a gene product specified by one of these prophages usually present in B. subtilis strain 168. Based on these results we conclude that the 45 kD protein is involved in recombination in B. subtilis.
利用二维凝胶电泳技术，将转化能力强的枯草芽孢杆菌菌株中的蛋白质与携带recE4突变的同基因重组缺陷型菌株中的蛋白质进行了比较。发现一种蛋白质（分子量45kD，等电点5.4）在recE4菌株中几乎不存在。这种45kD的蛋白质是一种主要存在于感受态培养物感受态部分的显著蛋白质。用紫外线照射或丝裂霉素C处理可显著刺激该蛋白质的合成，用萘啶酸处理也有一定程度的刺激。由于在治愈了SPβ并携带不可诱导的PBS X的菌株中也观察到了这种蛋白质，因此这种蛋白质不太可能是通常存在于枯草芽孢杆菌168菌株中的这些原噬菌体之一所指定的基因产物。基于这些结果，我们得出结论，45kD的蛋白质参与枯草芽孢杆菌的重组。
