枯草芽孢杆菌重组缺陷突变体的特性分析
Characterization of recombination-deficient mutants of Bacillus subtilis.
作者信息
Alonso J C, Tailor R H, Lüder G
机构信息
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.
出版信息
J Bacteriol. 1988 Jul;170(7):3001-7. doi: 10.1128/jb.170.7.3001-3007.1988.
Abstract
An isogenic set of "prophage-free," DNA repair-proficient and -deficient strains of Bacillus subtilis were characterized phenotypically. The mutant strains were provisionally classified into four categories on the basis of their sensitivity to DNA-damaging agents, their ability to release phage after lysogenization followed by damage to chromosomal DNA, and their impairment in genetic exchange. The properties of double Rec- mutants showed that recF and addA belong to different epistatic groups, whereas recF, recL, and recH fall into the same group. More than one pathway for genetic exchange might be operative in B. subtilis.
摘要
对一组同基因的“无原噬菌体”、DNA修复能力正常和缺陷的枯草芽孢杆菌菌株进行了表型特征分析。根据突变菌株对DNA损伤剂的敏感性、溶源化后损伤染色体DNA后释放噬菌体的能力以及它们在基因交换方面的缺陷，将这些突变菌株初步分为四类。双Rec-突变体的特性表明，recF和addA属于不同的上位性组，而recF、recL和recH属于同一组。枯草芽孢杆菌中可能存在不止一条基因交换途径。
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