Zhao Yu, van Hasselt Charles A, Woo John K S, Chen George G, Wong Yeuk O, Wang Li H, Leung Ping C
Department of Surgery, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong SAR, China.
Ann Allergy Asthma Immunol. 2005 Jul;95(1):79-85. doi: 10.1016/S1081-1206(10)61192-1.
The traditional Chinese herbal formula Cang Er Zi San has been used for the treatment of rhinitis, paranasal sinusitis, and allergic rhinitis for several centuries. However, its therapeutic mechanisms remain largely unclear.
To study the effects of Shi-Bi-Lin (SBL), a modified Cang Er Zi San formula, on cytokine release from and expressions in the human mast cell line (HMC-1).
The HMC-1 was preincubated with different concentrations of SBL extract solution 1 hour before being stimulated with 25 ng/mL of phorbol myristate acetate plus 2.5 x 10(-7)M calcium ionophore A23187 and then further incubated for 6, 12, and 24 hours, respectively. The cell culture supernatants were harvested, and the cytokines of interleukin 4 (IL-4), IL-6, IL-8, and tumor necrosis factor alpha (TNF-alpha) in the supernatants were measured by enzyme-linked immunosorbent assay. Furthermore, the total RNA of the cells was extracted, and the cytokines' messenger RNA expressions were examined using semiquantitative reverse transcriptase-polymerase chain reaction.
After different incubation periods at different concentrations, SBL could potently inhibit the cytokines of IL-4 and TNF-alpha and modestly affect IL-6 but not obviously affect IL-8 release from the HMC-1. However, no inhibitory effects were detected on the messenger RNA expressions of these cytokines.
These results demonstrate that SBL could modulate the mast cell-mediated hypersensitivity reaction in allergy. Inhibition of mast cell-derived IL-4 and TNF-alpha might explain the efficacy of SBL in treating allergic disease.
几个世纪以来，中药方剂苍耳子散一直用于治疗鼻炎、鼻窦炎和过敏性鼻炎。然而，其治疗机制仍不清楚。
研究苍耳子散改良方实鼻灵（SBL）对人肥大细胞系（HMC-1）细胞因子释放及表达的影响。
用25 ng/mL佛波酯肉豆蔻酸酯乙酸盐加2.5×10⁻⁷M钙离子载体A23187刺激HMC-1细胞前1小时，将其与不同浓度的SBL提取液预孵育，然后分别继续孵育6、12和24小时。收集细胞培养上清液，采用酶联免疫吸附测定法检测上清液中白细胞介素4（IL-4）、IL-6、IL-8和肿瘤坏死因子α（TNF-α）等细胞因子。此外，提取细胞的总RNA，采用半定量逆转录聚合酶链反应检测细胞因子的信使核糖核酸表达。
在不同浓度下经过不同孵育时间后，SBL能有效抑制HMC-1细胞释放IL-4和TNF-α，对IL-6有一定影响，但对IL-8释放无明显影响。然而，未检测到对这些细胞因子信使核糖核酸表达的抑制作用。
这些结果表明，SBL可调节肥大细胞介导的过敏超敏反应。抑制肥大细胞来源的IL-4和TNF-α可能解释了SBL治疗过敏性疾病的疗效。
