Schatzberg Scott J, Haley Nicholas J, Barr Stephen C, de Lahunta Alexander, Sharp Nicholas J H
Cornell University Hospital for Animals, Cornell University, Ithaca, NY, USA.
J Vet Intern Med. 2005 Jul-Aug;19(4):553-9. doi: 10.1892/0891-6640(2005)19[553:pcrsfd]2.0.co;2.
The objective of this investigation was to determine whether or not herpesvirus (herpes-), adenovirus (adeno-), or canine parvovirus DNA is present in the brains of dogs with necrotizing meningoencephalitis (NME), necrotizing leukoencephalitis (NLE), and granulomatous meningoencephalitis (GME). Paraffin-embedded brain specimens from 12 histopathologically confirmed dogs with NME, 3 with NLE, and 7 with GME were screened for viral DNA with degenerate herpes- and adenovirus polymerase chain reaction (PCR) and a canine parvovirus-specific PCR. Positive-control specimens included genomic viral DNA and paraffin-embedded tissues from dogs with confirmed herpes-, adeno-, or canine parvovirus infections. Herpes-, adeno-, or canine parvovirus DNA was amplified by PCR from the corresponding positive-control specimens. Negative controls included 7 dogs with various brain disorders and produced no viral amplicons. The 22 dogs with NME, NLE, and GME were negative for viral DNA. Additional studies testing for other viruses or inherited genetic mutations are warranted to gain insight into the etiologies of NME, NLE, and GME. We discuss potential etiologies and provide a clinical and histopathologic overview of these common canine encephalitides.
本研究的目的是确定坏死性脑膜脑炎（NME）、坏死性白质脑炎（NLE）和肉芽肿性脑膜脑炎（GME）患犬的大脑中是否存在疱疹病毒（疱疹 -）、腺病毒（腺 -）或犬细小病毒DNA。对12例经组织病理学确诊为NME的犬、3例NLE的犬和7例GME的犬的石蜡包埋脑标本进行了检测，采用简并疱疹病毒和腺病毒聚合酶链反应（PCR）以及犬细小病毒特异性PCR筛选病毒DNA。阳性对照标本包括来自确诊为疱疹病毒、腺病毒或犬细小病毒感染犬的基因组病毒DNA和石蜡包埋组织。通过PCR从相应的阳性对照标本中扩增出疱疹病毒、腺病毒或犬细小病毒DNA。阴性对照包括7只患有各种脑部疾病的犬，未产生病毒扩增产物。22只患有NME、NLE和GME的犬的病毒DNA检测结果均为阴性。有必要进行其他病毒检测或遗传性基因突变检测的进一步研究，以深入了解NME、NLE和GME的病因。我们讨论了潜在病因，并提供了这些常见犬类脑炎的临床和组织病理学概述。
