评价脑组织或脑脊液中广泛反应性聚合酶链反应在犬神经疾病中的埃立克体、无形体、斑点热群立克次体、巴尔通体和螺旋体属（109 例）。
Evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for Ehrlichia, Anaplasma, spotted fever group Rickettsia, Bartonella, and Borrelia species in canine neurological diseases (109 cases).
机构信息
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
出版信息
J Vet Intern Med. 2010 Mar-Apr;24(2):372-8. doi: 10.1111/j.1939-1676.2009.0466.x.
BACKGROUND
Vector-transmitted microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, Bartonella, and Borrelia are commonly suspected in dogs with meningoencephalomyelitis (MEM), but the prevalence of these pathogens in brain tissue and cerebrospinal fluid (CSF) of dogs with MEM is unknown.
HYPOTHESIS/OBJECTIVES: To determine if DNA from these genera is present in brain tissue and CSF of dogs with MEM, including those with meningoencephalitis of unknown etiology (MUE) and histopathologically confirmed cases of granulomatous (GME) and necrotizing meningoencephalomyelitis (NME).
ANIMALS
Hundred and nine dogs examined for neurological signs at 3 university referral hospitals.
METHODS
Brain tissue and CSF were collected prospectively from dogs with neurological disease and evaluated by broadly reactive polymerase chain reaction (PCR) for Ehrlichia, Anaplasma, Spotted Fever Group Rickettsia, Bartonella, and Borrelia species. Medical records were evaluated retrospectively to identify MEM and control cases.
RESULTS
Seventy-five cases of MUE, GME, or NME, including brain tissue from 31 and CSF from 44 cases, were evaluated. Brain tissue from 4 cases and inflammatory CSF from 30 cases with infectious, neoplastic, compressive, vascular, or malformative disease were evaluated as controls. Pathogen nucleic acids were detected in 1 of 109 cases evaluated. Specifically, Bartonella vinsonii subsp. berkhoffii DNA was amplified from 1/6 dogs with histopathologically confirmed GME.
CONCLUSION AND CLINICAL IMPORTANCE
The results of this investigation suggest that microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, and Borrelia are unlikely to be directly associated with canine MEM in the geographic regions evaluated. The role of Bartonella in the pathogenesis of GME warrants further investigation.
背景
埃立克体属、无形体属、立克次体属、巴尔通体属和伯氏疏螺旋体属的经向量传播的微生物通常被怀疑与患有脑脊髓炎（MEM）的犬有关，但这些病原体在患有 MEM 的犬的脑组织和脑脊液（CSF）中的流行情况尚不清楚。
假设/目的：确定这些属的 DNA 是否存在于患有 MEM 的犬的脑组织和 CSF 中，包括患有病因不明的脑膜脑炎（MUE）以及经组织病理学证实的肉芽肿性（GME）和坏死性脑脊髓炎（NME）的病例。
动物
在 3 所大学转诊医院检查有神经症状的 109 只犬。
方法
前瞻性采集患有神经疾病犬的脑组织和 CSF，并用广泛反应的聚合酶链反应（PCR）对埃立克体属、无形体属、斑点热群立克次体属、巴尔通体属和伯氏疏螺旋体属进行检测。回顾性评估病历以确定 MEM 和对照病例。
结果
评估了 75 例 MUE、GME 或 NME，包括 31 例脑组织和 44 例 CSF。评估了 4 例有感染性、肿瘤性、压迫性、血管性或畸形性疾病的病例的脑组织和 30 例有炎症性 CSF。在 109 例评估的病例中，有 1 例检测到病原体核酸。具体而言，从 6 例经组织病理学证实的 GME 犬中扩增出了巴尔通体属 vinsonii 亚种 berkhoffii 的 DNA。
结论和临床意义
本研究结果表明，在评估的地理区域，埃立克体属、无形体属、立克次体属和伯氏疏螺旋体属的微生物不太可能与犬 MEM 直接相关。巴尔通体属在 GME 发病机制中的作用值得进一步研究。
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