Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
J Vet Intern Med. 2012 Jul-Aug;26(4):962-8. doi: 10.1111/j.1939-1676.2012.00954.x. Epub 2012 Jun 11.
Granulomatous meningoencephalomyelitis (GME) and necrotizing meningoencephalitis (NME) are common inflammatory conditions of the central nervous system of dogs. Infectious pathogens, particularly viruses, are suspected to contribute to the etiopathogenesis of GME and NME.
Broadly reactive PCR might aid in the identification of infectious agents in GME and NME.
Sixty-eight client-owned dogs evaluated by necropsy at 1 university referral hospital.
A mixed prospective/retrospective case-control study was performed. Brain tissue prospectively collected at necropsy from GME, NME, and control cases was evaluated by broadly reactive polymerase chain reaction (PCR) for adenoviruses, bunyaviruses, coronaviruses, enteroviruses, flaviviruses, herpesviruses, paramyxoviruses, and parechoviruses. In addition, these tissues were retrospectively evaluated for the presence of mycoplasmas by PCR, culture, and immunohistochemistry (IHC).
Brain tissue was collected from 11 GME and 27 NME cases and 30 controls. Viral nucleic acids were not identified in the 6 GME cases, 25 NME cases, and 2 controls evaluated by viral PCR. Mycoplasma canis was identified by Mycoplasma genus PCR in 1/5 GME and 4/25 NME cases and subsequently was cultured from 4/5 GME and 4/8 NME cases as well as 2/9 controls. The IHC did not detect M. canis in any of the 11 GME and 27 NME cases or 14 controls evaluated with strain PG14 polyclonal antiserum.
The negative results suggest that viral pathogens are not common in the brain tissue of dogs with GME and NME. Further investigation is warranted to determine the importance of M . canis in cases of GME and NME.
肉芽肿性脑膜脑炎（GME）和坏死性脑膜脑炎（NME）是犬中枢神经系统常见的炎症性疾病。感染性病原体，特别是病毒，被怀疑与 GME 和 NME 的病因发病机制有关。
广谱聚合酶链反应（PCR）可能有助于鉴定 GME 和 NME 中的感染因子。
在 1 所大学转诊医院进行尸检评估的 68 只患犬。
进行了一项混合前瞻性/回顾性病例对照研究。通过广谱聚合酶链反应（PCR）对 GME、NME 和对照病例的尸检时采集的脑组织进行腺病毒、布尼亚病毒、冠状病毒、肠道病毒、黄病毒、疱疹病毒、副粘病毒和 parechovirus 的检测。此外，还通过 PCR、培养和免疫组织化学（IHC）对这些组织中支原体的存在情况进行了回顾性评估。
从 11 例 GME 和 27 例 NME 病例以及 30 例对照中采集了脑组织。通过病毒 PCR 未在 6 例 GME 病例、25 例 NME 病例和 2 例对照中检测到病毒核酸。1/5 GME 和 4/25 NME 病例中通过支原体属 PCR 鉴定出犬支原体，随后从 4/5 GME 和 4/8 NME 病例以及 2/9 对照中培养出支原体。用 PG14 多克隆抗血清进行 IHC 检测未在 11 例 GME 和 27 例 NME 病例或 14 例对照中检测到 M. canis。
阴性结果表明，病毒病原体在 GME 和 NME 患犬的脑组织中并不常见。需要进一步研究以确定犬支原体在 GME 和 NME 病例中的重要性。
