Kirkeby O J, Nordsletten L, Skjeldal S
Institute for Surgical Research, University of Oslo, Norway.
Acta Orthop Scand. 1992 Jun;63(3):318-22. doi: 10.3109/17453679209154791.
We studied healing of allogeneic and syngeneic cortical tibial segment grafts in athymic and normal rats. After 3, 6, and 12 weeks, the weight, circulation, and mineralization rate of the healing segment, and mechanical strength and stiffness of the healing tibia were measured. There were no differences between allogeneic and syngeneic grafts in athymic and normal animals at 3 or 6 weeks. After 12 weeks, the vascularization and mineralization of the grafts, but not of the surrounding callus, were smaller in the allogeneic grafts in the normal recipients than in the other groups. Also after 12 weeks, the stiffness of the healing tibiae was less in allogeneic grafts in normal recipients than in the other groups. The strength of the allogeneic grafts was less than the strength of the syngeneic grafts in both athymic and normal recipients. This suggests that T-cell-mediated rejection is responsible for decreased vascularization and mineralization of allogeneic bone and that the difference in strength between allogeneic and syngeneic grafts is not due to T-lymphocyte graft rejection.
我们研究了无胸腺大鼠和正常大鼠同种异体及同基因皮质胫骨段移植物的愈合情况。在3周、6周和12周后，测量愈合段的重量、血液循环和矿化率，以及愈合胫骨的机械强度和刚度。在3周或6周时，无胸腺动物和正常动物的同种异体移植物与同基因移植物之间没有差异。12周后，正常受体中同种异体移植物的血管化和矿化程度低于其他组，但周围骨痂的血管化和矿化程度并非如此。同样在12周后，正常受体中同种异体移植物愈合胫骨的刚度低于其他组。在无胸腺受体和正常受体中，同种异体移植物的强度均低于同基因移植物的强度。这表明T细胞介导的排斥反应是同种异体骨血管化和矿化减少的原因，同种异体移植物和同基因移植物之间的强度差异并非由于T淋巴细胞移植物排斥反应。
