Aimi Tadanori, Yoshida Rie, Ishikawa Mariko, Bao Dapeng, Kitamoto Yutaka
Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori-shi, Tottori, 680-8553 Japan.
Curr Genet. 2005 Sep;48(3):184-94. doi: 10.1007/s00294-005-0012-7. Epub 2005 Oct 12.
In the current studies, we sequenced and characterized the gene for the homeodomain protein (hox1) in a bipolar mushroom, Pholiota nameko, which is a putative homologue of A mating type genes in the tetrapolar basidiomycete, Coprinopsis cinerea. We also sequenced and characterized the gene for the pheromone receptor (rcb1) in P. nameko, which is a putative homologue of the B mating type genes in C. cinerea. Restriction fragment length polymorphism (RFLP) and linkage analyses indicated that the both genes are present as a single locus on the different chromosome. Moreover, in P. nameko, the hox1 gene was mapped to the A mating type locus in linkage group I. However, rcb1 was not linked to the A mating type locus and was mapped to the other linkage group. These results strongly suggest that hox1 regulates with incompatibility in the bipolar mushroom, and that rcb1 may not affect the mating function in P. nameko. This is the first report regarding the structure of the mating type genes in bipolar mushrooms.
在当前的研究中，我们对双极性蘑菇滑菇中的同源异型域蛋白（hox1）基因进行了测序和特征分析，该基因被认为是四极性担子菌灰盖鬼伞中A交配型基因的同源物。我们还对滑菇中的信息素受体（rcb1）基因进行了测序和特征分析，该基因被认为是灰盖鬼伞中B交配型基因的同源物。限制性片段长度多态性（RFLP）和连锁分析表明，这两个基因均作为单一位点存在于不同的染色体上。此外，在滑菇中，hox1基因被定位到连锁群I中的A交配型位点。然而，rcb1与A交配型位点不连锁，而是被定位到另一个连锁群。这些结果有力地表明，hox1在双极性蘑菇中调控不亲和性，并且rcb1可能不影响滑菇的交配功能。这是关于双极性蘑菇交配型基因结构的首次报道。
