单核细胞增生李斯特菌铁蛋白可抵御多种应激，且是毒力所必需的。
Listeria monocytogenes ferritin protects against multiple stresses and is required for virulence.
作者信息
Dussurget Olivier, Dumas Emilie, Archambaud Cristel, Chafsey Ingrid, Chambon Christophe, Hébraud Michel, Cossart Pascale
机构信息
Unité des Interactions Bactéries-Cellules, INSERM U604, INRA USC2020, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France.
出版信息
FEMS Microbiol Lett. 2005 Sep 15;250(2):253-61. doi: 10.1016/j.femsle.2005.07.015.
In this study, the role of Listeria monocytogenes ferritin was investigated. The fri gene encoding the ferritin was deleted and the phenotype of the mutant was analyzed demonstrating that ferritin is necessary for optimal growth in minimal medium in both presence and absence of iron, as well as after cold- and heat-shock. We also showed that ferritin provides protection against reactive oxygen species and is essential for full virulence of L. monocytogenes. A comparative proteomic analysis revealed an effect of the fri deletion on the levels of listeriolysin O and several stress proteins. Together, our study demonstrates that fri has multiple roles that contribute to Listeria virulence.
在本研究中，对单核细胞增生李斯特菌铁蛋白的作用进行了研究。编码铁蛋白的fri基因被删除，并对突变体的表型进行了分析，结果表明，无论有无铁，以及在冷休克和热休克后，铁蛋白对于在基本培养基中的最佳生长都是必需的。我们还表明，铁蛋白可提供针对活性氧的保护作用，并且对于单核细胞增生李斯特菌的完全毒力至关重要。一项比较蛋白质组学分析揭示了fri缺失对溶血素O和几种应激蛋白水平的影响。总之，我们的研究表明fri具有多种有助于李斯特菌毒力的作用。
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