Domiati-Saad Rana, Scheuermann Richard H
Department of Pathology, Baylor University Medical Center, Dallas, TX 75246, USA.
Clin Chim Acta. 2006 Jan;363(1-2):197-205. doi: 10.1016/j.cccn.2005.05.049. Epub 2005 Aug 11.
Molecular diagnostics plays an important role in the diagnosis and clinical management of a wide array of infectious diseases.
Advances in molecular technology and methods of detecting nucleic acid sequences have revolutionized the field of virology. These developments are reflected by the rapid diagnosis and monitoring of viral agents as well as assessment of clinical disease associated with viral infections. In addition to many commercially available molecular based assays, many laboratories offer in-house developed assays for a variety of viral targets. The introduction of real-time PCR technology has made a large impact on virology testing.
The role of real-time PCR for the diagnosis of viral infections is enhanced by the accuracy, rapidity and ability to quantitate viral target sequences.
分子诊断在多种传染病的诊断和临床管理中发挥着重要作用。
分子技术及核酸序列检测方法的进展彻底改变了病毒学领域。这些进展体现在病毒病原体的快速诊断和监测以及与病毒感染相关的临床疾病评估方面。除了许多市售的基于分子的检测方法外，许多实验室还提供针对多种病毒靶点的内部开发检测方法。实时PCR技术的引入对病毒学检测产生了重大影响。
实时PCR在病毒感染诊断中的作用因其准确性、快速性以及定量病毒靶序列的能力而得到增强。
