Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
Nano Lett. 2012 Mar 14;12(3):1722-8. doi: 10.1021/nl300372a. Epub 2012 Feb 24.
A new single-molecule approach for rapid and purely electronic discrimination among similar genes is presented. Combining solid-state nanopores and γ-modified synthetic peptide nucleic acid probes, we accurately barcode genes by counting the number of probes attached to each gene and measuring their relative spacing. We illustrate our method by sensing individual genes from two highly similar human immunodeficiency virus subtypes, demonstrating feasibility of a novel, single-molecule diagnostic platform for rapid pathogen classification.
一种新的单分子方法可用于快速且纯粹地对相似基因进行电子区分。我们结合固态纳米孔和 γ 修饰的合成肽核酸探针，通过计算每个基因上连接的探针数量并测量它们的相对间距，精确地对基因进行标记。我们通过检测两种高度相似的人类免疫缺陷病毒亚型中的单个基因来说明我们的方法，这证明了用于快速病原体分类的新型单分子诊断平台具有可行性。
