Couto S Rodríguez, Rosales E, Sanromán M A A
Department of Chemical Engineering, University of Vigo, 36200 Vigo, Spain.
Chemosphere. 2006 Mar;62(9):1558-63. doi: 10.1016/j.chemosphere.2005.06.042. Epub 2005 Aug 11.
The present paper studies the decolourization of different synthetic dyes (Indigo Carmine, Bromophenol Blue, Methyl Orange and Poly R-478) by the white-rot fungus Trametes hirsuta at bioreactor scale under solid-state conditions, operating with ground orange peelings as a support-substrate. Dye decolourization was performed in both batch and continuous mode. Batch cultivation led to high decolourization percentages in a short time (100% for Indigo Carmine in 3h and 85% for Bromophenol Blue in 7 h). As for continuous cultivation, different hydraulic retention times (HRT) were studied (0.8, 1, 1.5 and 3d). The highest decolourization percentages were obtained operating at a HRT of 3d, especially for the dyes Methyl Orange and Poly R-478 (81.4% and 46.9%, respectively). This is a very interesting result, since there are few studies dealing with the continuous decolourization of dyes at bioreactor scale by fungal laccases.
本文研究了在固态条件下，以磨碎的橙皮为支撑底物，在生物反应器规模下，白腐真菌糙皮侧耳对不同合成染料（靛蓝胭脂红、溴酚蓝、甲基橙和聚R - 478）的脱色情况。染料脱色以分批和连续模式进行。分批培养在短时间内导致了高脱色率（靛蓝胭脂红在3小时内达到100%，溴酚蓝在7小时内达到85%）。至于连续培养，研究了不同的水力停留时间（HRT）（0.8、1、1.5和3天）。在3天的HRT下操作获得了最高的脱色率，特别是对于甲基橙和聚R - 478染料（分别为81.4%和46.9%）。这是一个非常有趣的结果，因为很少有关于真菌漆酶在生物反应器规模下对染料进行连续脱色的研究。
