Gualtieri Maurizio, Rigamonti Laura, Galeotti Valentina, Camatini Marina
Department of Environmental Science, University of Milano-Bicocca, Piazza della Scienza 1, 20126 Milano, Italy.
Toxicol In Vitro. 2005 Oct;19(7):1001-8. doi: 10.1016/j.tiv.2005.06.038. Epub 2005 Aug 11.
TD, produced by tire wear, is a significant constituent of PM(10) in urban areas where traffic related emissions are predominant. TD contains a lot of chemicals which can affect human respiratory system and it has received little attention until now, even the toxicity of PM has been extensively documented. A549 cells, a human alveolar lung cells, were exposed for 24, 48, 72 h to 10, 50, 60, 75 microg/ml of TD organic extract. MTT and Trypan Blue assays were used to evaluate cytotoxicity and Comet Assay to evidence DNA damage. TD extracts induced a dose-dependent increase in cell mortality and DNA damage. A significant toxicity was observed when cells were exposed to 60 microg/ml for 72 h. Moreover cell morphology observed at ultra structural level, was severely affected at the highest dose.
由轮胎磨损产生的轮胎磨损颗粒（TD）是交通相关排放占主导的城市地区细颗粒物（PM10）的重要组成部分。TD含有许多会影响人体呼吸系统的化学物质，直到现在它都很少受到关注，即便细颗粒物的毒性已有大量记录。人肺泡肺细胞A549细胞分别暴露于10、50、60、75微克/毫升的TD有机提取物中24、48、72小时。采用MTT法和台盼蓝试验评估细胞毒性，彗星试验检测DNA损伤。TD提取物导致细胞死亡率和DNA损伤呈剂量依赖性增加。当细胞暴露于60微克/毫升72小时时，观察到显著毒性。此外，在超微结构水平观察到的细胞形态在最高剂量下受到严重影响。
