Deng Peigang, Lee Yi-Kuen, Cheng Ping
Department of Mechanical Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon.
Biosens Bioelectron. 2006 Feb 15;21(8):1443-50. doi: 10.1016/j.bios.2005.06.007. Epub 2005 Aug 11.
Two-dimensional micro-bubble actuator arrays were developed and studied in detail to enhance the hybridization kinetics of a DNA micro-biosensor. The hybridization between a molecular beacon, a kind of oligonucleotide probe, and its complement was investigated in a millimeter-sized PDMS based reaction chamber, where various 2D micro-heater arrays were distributed on the bottom for micro-bubble generation. The hybridization assay without the micro-bubble actuation revealed that the fluorescence increased fast at the beginning and slowed down after that. However, a uniform fluorescence increase was observed when periodic micro-bubble agitation was introduced in the static hybridization solution. A comparison of hybridization assays with and without micro-bubble agitation revealed that the hybridization time could be effectively shortened by 33% with 10 cycles of micro-bubble agitation from a 2 x 1 bubble actuator array, and by 43% with 10 cycles of micro-bubble agitation from a 2 x 2 bubble actuator array.
为提高DNA微生物传感器的杂交动力学，开发并详细研究了二维微气泡致动器阵列。在基于聚二甲基硅氧烷（PDMS）的毫米级反应室中，研究了分子信标（一种寡核苷酸探针）与其互补物之间的杂交，反应室底部分布有各种二维微加热器阵列以产生微气泡。未进行微气泡驱动的杂交试验表明，荧光在开始时快速增加，之后减慢。然而，当在静态杂交溶液中引入周期性微气泡搅拌时，观察到荧光均匀增加。有微气泡搅拌和无微气泡搅拌的杂交试验比较表明，来自2×1气泡致动器阵列的10个周期微气泡搅拌可有效缩短杂交时间33%，来自2×2气泡致动器阵列的10个周期微气泡搅拌可有效缩短杂交时间43%。
