Guerasimova Anna, Nyarsik Lajos, Liu Jian-Ping, Schwartz Regine, Lange Matthias, Lehrach Hans, Janitz Michal
Max Planck Institute for Molecular Genetics, Berlin, Germany.
Biomol Eng. 2006 Mar;23(1):35-40. doi: 10.1016/j.bioeng.2005.09.005. Epub 2005 Nov 18.
An assay for the fluorescent detection of short oligonucleotide probe hybridization in miniaturized high-density array platforms is presented. It combines hybridization in solution with real-time fluorescent detection, which involves measurement of fluorescence increase by means of an induced fluorescence resonance energy transfer. The feasibility of this approach using DNA or RNA as a target, and short DNA- as well as LNA (locked nucleic acid)-modified oligonucleotides as probes is shown. The presented approach could potentially contribute to a significant increase in the throughput of large-scale genomic applications, such as oligofingerprinting and genotyping, and also reduce material consumption.
本文介绍了一种用于在小型化高密度阵列平台中对短寡核苷酸探针杂交进行荧光检测的分析方法。它将溶液中的杂交与实时荧光检测相结合，实时荧光检测通过诱导荧光共振能量转移来测量荧光增强。结果表明了该方法以DNA或RNA为靶标，使用短DNA修饰的寡核苷酸以及LNA（锁核酸）修饰的寡核苷酸作为探针的可行性。所提出的方法可能会显著提高大规模基因组应用（如寡核苷酸指纹图谱分析和基因分型）的通量，同时还能减少材料消耗。
