Huebert Dana J, Bernstein Bradley E
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Curr Opin Genet Dev. 2005 Oct;15(5):476-81. doi: 10.1016/j.gde.2005.08.001.
With the availability of complete genome sequences for a number of organisms, a major challenge has become to understand how chromatin and its epigenetic modifications regulate genome function. High-throughput microarray and sequencing technologies are being combined with biochemical and immunological enrichment methods to obtain genome-scale views of chromatin in a variety of organisms. The data pinpoint novel, genomic elements and expansive chromatin domains, and offer insight into the functions of histone modifications. In parallel, state-of-the-art imaging techniques are being used to investigate higher-order chromatin organization, and are beginning to bridge our understanding of chromatin biology with that of chromosome structure.
随着多种生物体全基因组序列的可得性，一个主要挑战已变为理解染色质及其表观遗传修饰如何调控基因组功能。高通量微阵列和测序技术正与生化及免疫富集方法相结合，以获得多种生物体染色质的基因组规模视图。这些数据确定了新的基因组元件和扩展的染色质结构域，并为组蛋白修饰的功能提供了见解。同时，最先进的成像技术正被用于研究高阶染色质组织，并开始将我们对染色质生物学的理解与染色体结构的理解联系起来。
