Ren Chen, Zhang Liwen, Freitas Michael A, Ghoshal Kalpana, Parthun Mark R, Jacob Samson T
Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA.
J Am Soc Mass Spectrom. 2005 Oct;16(10):1641-53. doi: 10.1016/j.jasms.2005.06.001.
The acetylated isoforms of histone H4 from mouse lymphosarcoma cells treated with HDAC inhibitors trichostatin A (TSA) and depsipeptide (DDP) were separated by acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE), in-gel digested, and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). The acetylation pattern of histone H4 in mouse lymphosarcoma cells induced by TSA was established in which acetylation initially occurred at K16 followed by K12 and then K8 and/or K5. An identical order of acetylation was found for cells treated with DDP.
用组蛋白去乙酰化酶抑制剂曲古抑菌素A(TSA)和缩肽(DDP)处理的小鼠淋巴肉瘤细胞中的组蛋白H4乙酰化异构体,通过醋酸尿素-聚丙烯酰胺凝胶电泳(AU-PAGE)进行分离、胶内消化,然后通过基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)和液相色谱串联质谱(LC-MS/MS)进行分析。确定了TSA诱导的小鼠淋巴肉瘤细胞中组蛋白H4的乙酰化模式,其中乙酰化最初发生在K16,随后是K12,然后是K8和/或K5。在用DDP处理的细胞中发现了相同的乙酰化顺序。