Kiricuta I C, Rişcă R, Morariu V V
Morphol Embryol (Bucur). 1979 Oct-Dec;25(4):361-4.
By using rapid freezing-rapid thawing procedures on Ehrlich ascites tumor cells the authors succeeded in maintaining the viability of these cells, ascertained by two methods: a) trypan blue dye-exclusion test, and b) grafting in experimental animals of cells maintained for three days at -80 degrees C.
通过对艾氏腹水瘤细胞采用快速冷冻 - 快速解冻程序,作者成功维持了这些细胞的活力,这通过两种方法得以确定:a)台盼蓝拒染试验,以及b)将在 -80℃下保存三天的细胞接种到实验动物体内。