Zardi L, Cecconi C, Barbieri O, Carnemolla B, Picca M, Santi L
Cancer Res. 1979 Sep;39(9):3774-9.
In the present paper we have studied: (a) the concentration of fibronectin (FN) in plasma and in ascitic fluid of mice at different times after inoculation of Ehrlich ascites tumor cells; (b) the ability of Ehrlich ascites cells to synthesize and release FN; and (c) the localization of FN in Ehrlich ascites cells by immunofluorescence microscopy. It was found that (a) 4 to 5 days after inoculation of the tumor, the plasma concentration of FN was significantly higher [1.7 +/- 0.07% (S.E.) of total plasma protein] than that in the normal control mice (0.8 +/- 0.035); (b) FN is present in the ascitic fluid in all phases of tumor growth; (c) Ehrlich ascites cells cultured in vitro synthesize and release large amounts of FN in the culture medium; and (d) only about 1 to 2% of the tumor cells show a very small amount of FN, and this is mostly in the area of cell-cell contact.
在本论文中，我们研究了：（a）接种艾氏腹水癌细胞后不同时间小鼠血浆和腹水中纤连蛋白（FN）的浓度；（b）艾氏腹水细胞合成和释放FN的能力；以及（c）通过免疫荧光显微镜观察FN在艾氏腹水细胞中的定位。结果发现：（a）接种肿瘤4至5天后，血浆中FN的浓度[占总血浆蛋白的1.7±0.07%（标准误）]显著高于正常对照小鼠（0.8±0.035）；（b）在肿瘤生长的各个阶段腹水中均存在FN；（c）体外培养的艾氏腹水细胞在培养基中合成并释放大量FN；以及（d）仅约1%至2%的肿瘤细胞显示出极少量的FN，且大多位于细胞-细胞接触区域。
