Quitain Armando T, Oro Kazuyuki, Katoh Shunsaku, Moriyoshi Takashi
Research Institute for Solvothermal Technology, Takamatsu, Kagawa, Japan.
Bioresour Technol. 2006 Sep;97(13):1509-14. doi: 10.1016/j.biortech.2005.06.010. Epub 2005 Aug 15.
Recovery of the oil components of okara by ethanol-modified supercritical carbon dioxide extraction was investigated at 40-80 degrees C temperature and 12-30 MPa pressure. In a typical run (holding period of 2 h, continuous flow extraction of 5 h), results indicated that the oil component could be best obtained with a recovery of 63.5% at relatively low temperature of 40 degrees C and mild pressure of 20 MPa in the presence of 10 mol% EtOH as entrainer. Based on gas chromatography-mass spectrometry (GC-MS) analysis, the extracts consisted mainly of fatty acids and phytosterols, and traces of decadienal. Folin-Ciocalteau estimates of total phenols showed that addition of EtOH as entrainer increased the yield and the amount of phenolic compounds in the extracts. The amounts of two primary soy isoflavones, genistein and daidzein, in the extracts also increased with increasing amount of EtOH.
在40-80摄氏度的温度和12-30兆帕的压力下，研究了用乙醇改性的超临界二氧化碳萃取法回收豆渣中的油脂成分。在一次典型的实验中（保持时间为2小时，连续流动萃取5小时），结果表明，在相对较低的40摄氏度温度和20兆帕的温和压力下，以10摩尔%乙醇作为夹带剂时，可以最好地获得油脂成分，回收率为63.5%。基于气相色谱-质谱联用（GC-MS）分析，提取物主要由脂肪酸和植物甾醇组成，还有微量的癸二烯醛。福林-西奥尔特法对总酚的测定表明，添加乙醇作为夹带剂提高了提取物中酚类化合物的产量和含量。提取物中两种主要的大豆异黄酮染料木黄酮和大豆苷元的含量也随着乙醇含量的增加而增加。
