Fehér Erzsébet, Batbayar Bayarchimeg, Zelles Tivadar
Laboratory of Oral Morphology, Department of Anatomy, Histology, and Embryology, Semmelweis University, Budapest, Hungary.
Anat Rec A Discov Mol Cell Evol Biol. 2005 Sep;286(1):848-53. doi: 10.1002/ar.a.20215.
In our previous studies, a large number of substance P (SP)-immunoreactive (IR) nerve fibers were detected in the rat tongue and their number increased after inflammation, suggesting that these fibers might be involved in the axon reflex. Therefore, in this study, we have examined the different neuropeptide-containing nerve elements by light, electron, and confocal laser microscopy. SP, vasoactive intestinal polypeptide (VIP), and neuropeptide Y (NPY) IR varicose fibers were numerous compared with other ones. Small groups of ganglia with perikarya IR for SP, VIP, NPY, galanin, and somatostatin were observed. The SP-IR nerve cell bodies were mainly located in the tunica propria just below the epithelial lining. Double-labeling immunohistochemistry showed that the intrinsic SP-IR neurons did not colocalize VIP. The SP containing nerve terminals were observed in and below the epithelium as well as in very close contact to or making real synapses with other neurons in the intralingual ganglion. Our data confirmed the possibility of intrinsic sensory neurons, which might be the afferent branch of the intralingual reflex arch, while the VIP- and NPY-IR neurons located in the salivary glands, around the blood vessels, and in the muscle layer might constitute the efferent site of this reflex.
在我们之前的研究中，在大鼠舌部检测到大量P物质（SP）免疫反应性（IR）神经纤维，且炎症后其数量增加，提示这些纤维可能参与轴突反射。因此，在本研究中，我们通过光学显微镜、电子显微镜和共聚焦激光显微镜检查了不同的含神经肽神经成分。与其他神经纤维相比，SP、血管活性肠肽（VIP）和神经肽Y（NPY）免疫反应性曲张纤维数量众多。观察到小群神经节，其神经节细胞体对SP、VIP、NPY、甘丙肽和生长抑素呈免疫反应性。SP免疫反应性神经细胞体主要位于上皮衬里下方的固有层。双重标记免疫组织化学显示，固有SP免疫反应性神经元不与VIP共定位。在舌内神经节中，上皮内和上皮下以及与其他神经元非常紧密接触或形成真正突触的部位观察到含SP的神经终末。我们的数据证实了存在固有感觉神经元的可能性，其可能是舌内反射弧的传入分支，而位于唾液腺、血管周围和肌层的VIP和NPY免疫反应性神经元可能构成该反射的传出部位。
