Altdorfer K, Zelles T, Pongor E, Fehér Erzsébet
Department of Anatomy, Semmelweis University, Budapest, Hungary.
Acta Physiol Hung. 2012 Dec;99(4):479-88. doi: 10.1556/APhysiol.99.2012.4.12.
Lingual components of the autonomic nervous system are considered to be the most rostral portion of the enteric nervous system. Therefore our aim was to study the intrinsic nerve cell bodies and synapses using immunohisto-, immunocytochemical methods. Several small groups of ganglia with cell bodies immunoreactive (IR) for vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and substance P (SP) were observed just below the gustatory epithelium. A few somatostatin and galanin IR nerve cell bodies were also found. Many IR cell bodies were also demonstrated in the glands and next to blood vessels. Some of these cell bodies were multipolar and some of them were small neurons with an ovoid shape having only one process. Cell bodies positive for calcitonin gene-related peptide (CGRP) were detected neither in the superficial nor in the deep portion. Electronmicroscopical analysis demonstrated different IR nerve fibres having axo-somatic and axo-dendritic synapses with other immunonegative cells. In a few cases VIP IR nerve processes were found to synaptize with other VIP positive nerve cell bodies. These results support the existance of intralingual reflex in the tongue, where the ganglia might have an integrative role of the different neuropeptide containing nerve fibres.
自主神经系统的舌部成分被认为是肠神经系统最靠前的部分。因此，我们的目的是使用免疫组织化学和免疫细胞化学方法研究内在神经细胞体和突触。在味觉上皮下方观察到几小群神经节，其细胞体对血管活性肠肽（VIP）、神经肽Y（NPY）和P物质（SP）呈免疫反应性（IR）。还发现了一些生长抑素和甘丙肽IR神经细胞体。在腺体和血管旁也证实有许多IR细胞体。其中一些细胞体是多极的，还有一些是小神经元，呈卵形，只有一个突起。在浅层和深层均未检测到降钙素基因相关肽（CGRP）阳性的细胞体。电子显微镜分析显示不同的IR神经纤维与其他免疫阴性细胞形成轴-体和轴-树突触。在少数情况下，发现VIP IR神经突起与其他VIP阳性神经细胞体形成突触。这些结果支持舌内反射的存在，其中神经节可能对不同的含神经肽神经纤维具有整合作用。
