Kaur Navjot, Singh Jatinder, Kamboj Sukhdev Singh, Agrewala Javed N, Kaur Manpreet
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar-143005, India.
Protein Pept Lett. 2005 Aug;12(6):585-95. doi: 10.2174/0929866054395725.
Two mannose/glucose specific seed lectins were isolated from Parkia biglandulosa and Parkia roxburghii and were characterized w.r.t various physicochemical properties. Unlike other Parkia lectins a comparison of native and subunit molecular mass showed that both Parkia lectins were heterotetramers. Parkia biglandulosa lectin was found to be T-cell mitogen as revealed by IL-2 bioassay. These lectins showed anti-proliferative effect on two murine macrophage cancer cell lines i.e. P 388DI (50%) and J774 (70%). In addition Parkia roxburghii also inhibited proliferation of HB98 (65.47%), a B-cell hybridoma cell line.
从大果帕克木（Parkia biglandulosa）和罗氏帕克木（Parkia roxburghii）中分离出两种甘露糖/葡萄糖特异性种子凝集素，并对其各种物理化学性质进行了表征。与其他帕克木凝集素不同，天然和亚基分子量的比较表明，这两种帕克木凝集素均为异源四聚体。白细胞介素-2生物测定显示，大果帕克木凝集素是T细胞有丝分裂原。这些凝集素对两种小鼠巨噬细胞癌细胞系即P 388DI（50%）和J774（70%）具有抗增殖作用。此外，罗氏帕克木也抑制B细胞杂交瘤细胞系HB98（65.47%）的增殖。
