Suvachittanont W, Jaranchavanapet P
Faculty of Science, Prince of Songkla University, Hat Yai, Thailand.
Planta Med. 2000 Dec;66(8):699-704. doi: 10.1055/s-2000-9565.
Mitogenic activity of a lectin, purified from Parkia speciosa seeds, on the isolated peripheral blood lymphocytes taken from normal blood donors and patients with esophageal carcinoma was examined using [3H]thymidine incorporation. The lectin increases the incorporation of [3H]thymidine into DNA of human lymphocytes. The activity of the lectin increased as its concentration was increased and then declined once the concentration passed an optimum point. The stimulant effect was also expressed using a proliferation index (PI): the ratio of [3H]thymidine incorporated into lymphocytes in the presence and absence of the lectin. The mitogenic activity of the lectin is comparable to those of the known T-cell mitogens, such as concanavalin A, phytohaemagglutinin, and pokeweed mitogen. Only slightly less responsiveness was observed in the case of lymphocytes from esophageal cancer compared to lymphocytes from normal donors.
使用[3H]胸苷掺入法检测了从南洋楹种子中纯化得到的一种凝集素对正常献血者和食管癌患者外周血淋巴细胞的促有丝分裂活性。该凝集素可增加[3H]胸苷掺入人淋巴细胞DNA的量。凝集素的活性随其浓度增加而升高，一旦浓度超过最佳点则下降。刺激作用也用增殖指数（PI）表示：有凝集素和无凝集素时掺入淋巴细胞的[3H]胸苷的比率。该凝集素的促有丝分裂活性与已知的T细胞有丝分裂原如伴刀豆球蛋白A、植物血凝素和商陆有丝分裂原相当。与正常献血者的淋巴细胞相比，食管癌患者的淋巴细胞仅表现出稍低的反应性。
