Lesnik R H, Kligman L H, Kligman A M
Department of Dermatology, University of Pennsylvania, School of Medicine, Philadelphia 19104-6142.
Arch Dermatol Res. 1992;284(2):106-8. doi: 10.1007/BF00373379.
We have developed a murine model to measure the effects on sebaceous glands of ultraviolet (UV) radiation. Hairless mice were irradiated with Westinghouse FS-40 tubes filtered to attenuate the radiation below 290 nm. Emission was mainly in the UVB range (peak, 313nm). Single and multiple exposures were given with fractions or multiples of one minimal erythemal dose (MED). Biopsies, fixed for light microscopy, were stained with H & E. Under high power, sebocytes of 30 glands per specimen were counted and the means determined. A single exposure of 1 MED caused a significant increase in sebocyte count, as did thrice-weekly exposures to 0.5 MED for 3 weeks. One 3-MED exposure produced sebocyte necrosis, yet 30 exposures at 4 MED failed to ablate the glands. In both cases there was rebound enlargement which had not returned to control levels by the end of the studies (24-30 weeks). Prolonged irradiation produced maximum enlargement in a few weeks. Thus, in a manner similar to other skin components, the response of sebaceous glands to UV radiation is one of hyperplasia.
我们建立了一种小鼠模型，用于测量紫外线（UV）辐射对皮脂腺的影响。用经过过滤以衰减290nm以下辐射的西屋FS - 40灯管对无毛小鼠进行照射。发射主要在UVB范围内（峰值为313nm）。单次和多次照射分别给予一个最小红斑剂量（MED）的分数倍或倍数。用于光学显微镜检查的活检组织用苏木精和伊红染色。在高倍镜下，对每个标本的30个腺体的皮脂腺细胞进行计数并确定平均值。单次1 MED照射导致皮脂腺细胞计数显著增加，每周三次0.5 MED照射持续3周也有同样效果。一次3 MED照射导致皮脂腺细胞坏死，但4 MED照射30次未能消除腺体。在这两种情况下都有反弹增大，在研究结束时（24 - 30周）尚未恢复到对照水平。长时间照射在几周内产生最大程度的增大。因此，与其他皮肤成分类似，皮脂腺对紫外线辐射的反应是增生之一。
