Gilchrest B A, Stoff J S, Soter N A
J Invest Dermatol. 1982 Jul;79(1):11-5. doi: 10.1111/1523-1747.ep12510417.
In order to determine the effect of age on the capacity of human skin to mount an inflammatory response, the sunburn reaction was studied quantitatively in 4 subjects aged 22-26 yr and 7 subjects aged 62-86 yr. Buttock skin of each subject was exposed to 3 time his minimal erythema dose, using the Hanovia mercury vapor lamp; 1 micrometer histologic sections and determinations of histamine and prostaglandin E2 (PGE2) from suction blister aspirates were used to monitor the reaction. Clinically, erythema and edema were less (p less than .05) in irradiated skin of the old subjects during the first 24 hr. Nonirradiated skin contained less histamine and PGE2 in old adults (p less than .05), approximately 50% of young adult levels. Histamine levels rose early in the reaction and returned to baseline by 24 hr; 4 hr peak values averaged 9.2 ng/ml in old vs. 18.3 ng/ml in young adults. PGE2 rose more slowly in blister aspirates from old adults (P less than .05), but reached comparable peaks, 6-9 pg/T.V., in both groups at 24 hr. Biopsies of control skin from old adult specimens contained fewer mast cells and venules (P less than .01). At 4 and 24 hr, old specimens revealed fewer sunburn cells and less striking alterations of perivenular mast cells and endothelial cells, but at 72 hr these changes were more prominent than in young adult specimens. The data suggest that with advancing age the sunburn reaction is quantitatively reduced and evolves more slowly following a standardize ultraviolet exposure.
为了确定年龄对人体皮肤产生炎症反应能力的影响，对4名22 - 26岁的受试者和7名62 - 86岁的受试者的晒伤反应进行了定量研究。使用汉诺瓦汞蒸气灯，将每位受试者臀部皮肤暴露于其最小红斑量的3倍剂量下；采用1微米组织学切片以及对抽吸水疱液中组胺和前列腺素E2（PGE2）的测定来监测反应。临床上，在最初24小时内，老年受试者受照射皮肤的红斑和水肿较轻（p < 0.05）。老年成年人未受照射的皮肤中组胺和PGE2含量较少（p < 0.05），约为年轻成年人水平的50%。组胺水平在反应早期升高，并在24小时时恢复至基线；老年组4小时的峰值平均为9.2 ng/ml，而年轻成年人组为18.3 ng/ml。老年成年人水疱液中PGE2升高更为缓慢（P < 0.05），但在24小时时两组均达到相当的峰值，为6 - 9 pg/T.V.。老年成人标本的对照皮肤活检显示肥大细胞和小静脉较少（P < 0.01）。在4小时和24小时时，老年标本中的晒伤细胞较少，血管周围肥大细胞和内皮细胞的改变也不明显，但在72小时时，这些变化比年轻成人标本更为显著。数据表明，随着年龄的增长，在标准紫外线照射后，晒伤反应在数量上会减少，且发展更为缓慢。
